Flocculation protein structure and cell–cell adhesion mechanism in Saccharomyces cerevisiae
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- Goossens, K. & Willaert, R. Biotechnol Lett (2010) 32: 1571. doi:10.1007/s10529-010-0352-3
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Cell–cell adhesion occurs in a broad spectrum of biological processes, of which yeast flocculation is an area of interest for evolutionary scientists to brewers and winemakers. The flocculation mechanism is based on a lectin-carbohydrate interaction but is not yet fully understood, although the first model dates back to the 1950s. This review will update the current understanding of the complex mechanism behind yeast flocculation. Moreover, modern technologies to measure the forces involved in single carbohydrate-lectin interactions, are discussed. The Flo1 protein has been extensively described as the protein responsible for strong flocculation. Recently, more research has been directed to the detailed analysis of this flocculin. Due to the advances in the field of bioinformatics, more information about Flo1p could be obtained via structurally or functionally related proteins. Here, we review the current knowledge of the Flo1 protein, with a strong emphasis towards its structure.
KeywordsCell–cell adhesionFlo1pFlocculationFLO genesFlo proteinsSaccharomyces cerevisiae
The Flo adhesin family is involved in cell–cell and cell surface adhesion
A broad spectrum of biological processes requires controlled cell adhesion. In microbiology, this includes fungal, viral and bacterial infections or the formation of organised cell structures such as biofilms. Other examples are the assembly of tissues and the nervous system, cell culturing, embryonic development, cellular communication and tumor metastasis. Cell adhesion in general is commonly defined as the binding of a cell to a substrate, which can be another cell, a surface or an organic matrix. The process is regulated by specific cell adhesion molecules.
Many fungi contain a family of cell wall glycoproteins, called “adhesins” that confer unique adhesion properties (Teunissen and Steensma 1995; Guo et al. 2000; Hoyer 2001). These molecules are required for the interactions of fungal cells with each other (flocculation and filamentation) (Teunissen and Steensma 1995; Lo and Dranginis 1998; Guo et al. 2000; Vyas et al. 2003), inert surfaces such as agar and plastic (Gaur and Klotz 1997; Lo and Dranginis 1998; Reynolds and Fink 2001) and mammalian tissues (Cormack et al. 1999; Li and Palecek 2003). They are also crucial for the formation of fungal biofilms (Baillie and Douglas 1999; Reynolds and Fink 2001; Green et al. 2004).
The adhesin protein family in Saccharomyces cerevisiae strain S288C can be subdivided into two groups. The first group of proteins is encoded by genes including FLO1, FLO5, FLO9 and FLO10. These proteins are called flocculins (Caro et al. 1997) because they promote cell–cell adhesion by interacting with the cell wall of adjacent cells. This binding event leads to the formation of multicellular clumps (flocs), which sediment out of solution. The FLO1, FLO5, FLO9 and FLO10 genes share considerable sequence homology. The second group of the Flo family, including Flo11p, Fig2p and Aga1p, has quite unrelated amino acid sequences. These last two proteins are induced during mating (Roy et al. 1991; Erdman et al. 1998), while Flo11p is required for diploid pseudohyphal formation and haploid invasive growth (Lambrechts et al. 1996; Lo and Dranginis 1998). In haploid invasive growth, cells adhere to the agar surface, so that they do not wash off (Roberts and Fink 1994). In diploid pseudohyphal growth, cells adhere to each other after division and form long chains or filaments (Gimeno et al. 1992). When yeast cells are grown on semi-solid (0.3% agar) medium, they form “mats”: complex multicellular structures composed of yeast cells (Reynolds and Fink 2001; Reynolds et al. 2008). Yeast mat formation, as well as the attachment of cells to plastic surfaces, requires Flo11p.
These morphogenetic events (flocculation, filamentation and invasive growth) are tightly regulated (Verstrepen and Klis 2006; Fichtner et al. 2007; Dietvorst and Brandt 2008). Flocculation often occurs upon depletion of sugar during late-exponential or stationary phases of growth (Stewart and Russell 1981), whereas filamentation requires starvation for nitrogen (Gimeno et al. 1992; Braus et al. 2003; Granek and Magwene 2010). The FLO8 gene is a transcriptional activator of FLO1 (Kobayashi et al. 1996) and FLO11 (Rupp et al. 1999) and essential for their expression. In commonly used laboratory strains a nonsense mutation in the FLO8 gene leads to transcriptionally silencing of the FLO genes. Moreover, it was shown that one FLO gene may compensate for another in diverse morphogenetic events (Guo et al. 2000).
The dominant FLO1 gene was identified and localised at the very end of the right arm of chromosome I (Russell et al. 1980; Teunissen et al. 1993b). Flo1p is a cell wall protein responsible for flocculation of Flo1-expressing cells and its presence is directly related to the degree of flocculation (Bidard et al. 1995; Bony et al. 1997, 1998). The FLO5 gene is responsible for the strong flocculation of Flo5-expressing cells (Johnston and Reader 1983). This gene is not allelic with the FLO1 gene but shows 96% identity (Jin and Speers 1998). It is located on the very end of chromosome VIII (Teunissen et al. 1995). When the FLO5 gene is incorporated in a non-flocculent yeast strain, this strain acquires flocculation properties (Vezinhet et al. 1991; Bidard et al. 1995). Flo5p is, like Flo1p, a cell wall protein and the expression of Flo5p is correlated with the degree of flocculation (Bony et al. 1998). Not all yeast strains contain the dominant FLO9 gene, which is located on chromosome I, but at the other end of the chromosome, when compared to FLO1 (Teunissen and Steensma 1995). This gene shows 94% identity with FLO1 and 74% identity with the FLO5 gene (Bossier et al. 1997). The FLO10 gene product has 58% identity with FLO1 and is located at chromosome XI (Teunissen and Steensma 1995). Overexpressing the FLO10 gene in S. cerevisiae leads to weak flocculation compared to the overexpression of the FLO1 gene. Flo10p is also responsible for filamentation and the adhesion to agar or plastic (Guo et al. 2000). The Flo11p protein has the same domain structure as the other Flo proteins, but has a totally different amino acid sequence. The protein is responsible for flocculation, pseudohyphae formation, agar invasion, adhesion to substrates as well as for filamentation (Lambrechts et al. 1996; Lo and Dranginis 1996, 1998; Guo et al. 2000).
Generally, flocculation can be governed by the different FLO genes except by FLO11. But, when overexpressed, many of these proteins can substitute for each other in diverse morphogenic events: flocculation, mating, haploid invasion and filamentation (Guo et al. 2000). Indeed, yeast cells expressing Flo11p showed flocculation behaviour (Douglas et al. 2007) but the flocculation ability of yeast cells carrying the FLO11 gene depends on the strain in which this gene is expressed. The yeast strain S. cerevisiae var. diastaticus exhibits Flo11 dependent flocculation and biofilm formation but did not invade agar or form pseudohyphae, whereas the strain with the ∑1278b background required Flo11p to form pseudohyphae, invade agar, adhere to plastic and develop biofilms, but did not flocculate (Douglas et al. 2007). These results clearly show that adhesin properties are influenced by the yeast strain in which they are studied. Specific glycosylation of the adhesin, or auxiliary factors influencing adhesin conformation on the cell wall may thus be responsible for differences in biophysical properties. This has also been investigated by Govender et al. (2010). They explain that the adhesion phenotype after expressing individual FLO genes is different depending on the yeast strain in which they are expressed. This is due to the high allele heterogeneity of the FLO genes and to the fact that those genes are subjected to epigenetic regulation. So it can be concluded that care has to be taken when extrapolating data obtained in laboratory strains to industrial strains and that the optimization of the flocculation pattern of individual commercial strains will have to be based on a strain-by-strain approach (Govender et al. 2010).
Flo protein cell–cell adhesion mechanism
Flocculation was first defined as the phenomenon wherein yeast cells adhere in clumps, and sediment rapidly from the medium in which they are suspended (Stewart and Goring 1976). Further refinement led to the present definition: “flocculation is the asexual, reversible and calcium-dependent aggregation of yeast cells to form flocs containing thousands of cells that rapidly sediment to the bottom of the liquid growth substrate” (Stratford 1989; Bony et al. 1997). Other yeast strains, called ale yeasts, show a biofilm at the air–liquid interface at the end of the fermentation. The model for this biofilm formation is based on an increase in cell surface hydrophobicity and the fact that the multicellular aggregates effectively entrap carbon dioxide (Zara et al. 2005). The flocculation behaviour is of great importance for yeast cells as it is a way to escape from harsh conditions in the growth medium. It was suggested that cells in the middle of the floc could lyse and act as a source of new nutrients for the other cells (Stewart and Russell 1981; Iserentant 1996). Therefore, flocculation enhances the survival rate of yeast cells in starvation conditions.
In an attempt to elucidate the phenomenon of flocculation, Eddy and Rudin (1958) proposed the lectin hypothesis. In the presence of calcium, flocculins are able to bind highly branched mannose polymers located in the cell wall of adjacent cells (Eddy and Rudin 1958; Miki et al. 1982). Moreover, it was shown that flocculation could be reversibly inhibited by the presence of sugars (Taylor and Orton 1978; Miki et al. 1980; Nishihara and Toraya 1987; Stratford and Assinder 1991), which is validating the hypothesis. This flocculation model is now widely accepted.
The flocculation phenotypes of yeast strains can be divided into two groups: the Flo1-phenotype and the NewFlo-phenotype (Stratford and Assinder 1991; Sieiro et al. 1995). This partition is based on the type of carbohydrate that inhibits flocculation. Flocculation of the Flo1-phenotype can be inhibited by mannose, but not by glucose, maltose, sucrose or galactose. Therefore, Flo1p is considered as the most specific adhesion protein of the Flo-family (Kobayashi et al. 1998; Govender et al. 2008; Van Mulders et al. 2009). Flocculation of the NewFlo-phenotype can be inhibited by mannose, glucose, maltose and sucrose, but not by galactose. This flocculation phenotype is governed by a Flo1p homologue, named Lg-Flo1p. Considering the current research about the capacity of different sugars to inhibit the flocculation, the clear distinction of two flocculation phenotypes is questioned. Not all flocculation phenotypes can be classified in either the Flo-phenotype or the NewFlo-phenotype. For example, the expression of FLO5, FLO9 or FLO10 does not lead to a specific flocculation phenotype making them less selective adhesins. They were strongly inhibited by mannose but also weakly inhibited by a range of sugars (Van Mulders et al. 2009). Although, based on the sequence homology with Flo1p, it was found that Flo5p and Flo9p were exclusively mannose-binding flocculins (Teunissen and Steensma 1995). More detailed research about the inhibition of flocculation by sugars has been performed. Miki et al. have quantified the inhibition of flocculation (1980). Up to 500 mM d-mannose was required to completely inhibit flocculation. Later, the sugars were ranked according to their inhibition capacity (Smit et al. 1992). It was shown that α-d-mannose could disrupt the flocs at a concentration of only 25 mM. This experiment has been repeated recently using a yeast strain overexpressing Flo1p (Van Mulders et al. 2009). It was observed that the flocculation mediated by Flo1p was only inhibited by mannose at the high concentration of 1 M.
More detailed research regarding the flocculation of yeast cells has been performed recently. A high concentration of flocculins is found on the yeast cell surface (Bony et al. 1997). Where they can all interact with mannan chains of other cells, leading to multipoint attachments between yeast cells. When one interaction is in the range of millimolar affinities, it becomes micromolar affinity with two attachments. In this way the interaction strength of flocculation is increased dramatically (Dranginis et al. 2007). The first and only quantitative measurement of the affinity between a Flo protein and a carbohydrate was done for the interaction of Lg-Flo1p to mannose. The value for the dissociation constant was calculated to be 0.77 mM (Groes et al. 2002). Compared to the affinity of other lectin carbohydrate interactions, this is indeed a very low value. This two-way interaction has been confirmed by looking carefully at the cells part of a floc resulting from multiple stresses. While analysing the flocs, it was observed that most of the cells were FLO1-expressing cells. In other words, FLO1-expressing cells preferentially aggregate with other FLO1 expressers (Smukalla et al. 2008). This was explained by the fact that the two-way interaction, which occurs when two FLO1-expressers are binding, is stronger than the one-way interaction of a FLO1-expresser with a non-FLO1-expresser. A more striking observation has been described about the Flo11 proteins. When secreted Flo11p was covalently attached to microscopic beads, it conferred the ability to specifically bind to S. cerevisiae var. diastaticus, but not to ∑1278b cells, which do not flocculate. They were not able to bind cells from another yeast strain expressing Flo11p or cells with a deletion of FLO11 (Douglas et al. 2007). This Flo11p–Flo11p interaction points to a homotypic adhesive mechanism. A further quantitative investigation on the molecular level of the self-binding properties of Flo11p and the other Flo proteins is necessary to complete the flocculation mechanism.
Structural features of S. cerevisiae flocculins
Modular configuration of the Flo proteins
The C-terminal domain contains a glycosylphosphatidylinositol (GPI)-attachment site. The adhesins are covalently linked to the β-1,6-glucans of the yeast cell wall through the GPI-remnant (De Nobel and Lipke 1994; Caro et al. 1997; Hamada et al. 1998a, b; De Groot et al. 2003).
The N-terminal domain is the lectin domain, which interacts with mannose residues. This interaction is responsible for flocculation. This was discovered by expressing a truncated form of Flo1p (deletion of amino acids 50–278). It was observed that this truncated protein could not trigger flocculation (Bony et al. 1997). These results are in agreement with those of Kobayashi et al. (1998), who also reported that the N-terminal region of Flo1p contains the sugar recognition domain. Indeed, the replacement of the N-terminus of the Flo1 protein by the corresponding region of the Lg-Flo1 protein, caused conversion of the Flo1 flocculation phenotype to the NewFlo flocculation phenotype (Kobayashi et al. 1998).
The Flo protein at molecular level
The amino acids that are responsible for the carbohydrate recognition in Flo1p and Lg-Flo1p, have also been described. It was suggested that the tryptophan residue on position 228 is involved in mannose-recognition in the Flo1 flocculation phenotype. This tryptophan 228 must be replaced by leucine to produce a flocculation phenotype inhibited by glucose. The following model for sugar recognition was proposed: tryptophan 228 in Flo1p recognises the C2-hydroxyl group of mannose but does not recognise the C2-hydroxyl group of glucose. In the Lg-Flo1 protein, a leucine instead of a tryptophan at position 228 is required for the NewFlo flocculation phenotype. Also position 226 affects the flocculation phenotype: for NewFlo-type flocculation a glycine or arginine is needed at that position. Generally, the carbohydrate binding activity of Flo1p can be attributed to the VSWGT motif, a pentapeptide encompassing amino acids 226–230 (Kobayashi et al. 1998).
Recently, an EYDGA pentapeptide motif belonging to the PA14 domain was identified. The pentapeptide is involved in sugar recognition in the N-terminal domain of the Epa1p (epithelial adhesin) from Candida glabrata. This can be compared to the VSWGT pentapeptide in Flo1p (Zupancic et al. 2008). A multiple sequence alignment of various PA14 domains showed convincingly that the VSWGT motif of Flo1p and the EYDGA motif of Epa1p are found in precisely the same position, within a hypervariable region of PA14 (Rigden et al. 2004). The VSWGT/KVLAR motif of Flo1p/Lg-Flo1p, and the EYDGA motif of Epa1p, correspond to a surface loop between two β-strands (strands 9 and 10) in the anthrax toxin PA domain structure (Petosa et al. 1997) (see the blue loop in Fig. 3b).
The FLO genes posses internal tandem repeats located in the central region, that function as interchangeable modules (Watari et al. 1994). During DNA replication, the presence of internal tandem repeats is responsible for recombination events. Therefore, yeast cells show a highly variable adhesion phenotype as they are able to quickly adapt their adhesion properties to new environments. The removal or addition of repeat units results in a longer or shorter adhesin. This is immediately reflected in its flocculation behaviour: a longer adhesin showing a stronger flocculation phenotype. This can be explained by the fact that the N-terminal domain should be exposed clearly to the neighbouring cells to allow flocculation (Frieman et al. 2002; Frieman and Cormack 2004; Verstrepen et al. 2005). Surprisingly, Liu et al. suggest that the deletion of tandem repeats can cause the conversion from Flo1- to NewFlo1-phenotype (Liu et al. 2007a, b). Moreover, for the Flo11 protein, the length of the gene affects the biofilm-forming ability of the cells expressing Flo11p. This was demonstrated by cloning two alleles of the FLO11 gene with different sizes (3.1 and 5 kb) into S. cerevisiae strain BY4742 and analysing the corresponding phenotype (Zara et al. 2009). This is not in agreement with what is known so far about the specificity of the flocculin, which is believed to be on the N-terminal domain only (Bony et al. 1997; Kobayashi et al. 1998; Van Mulders et al. 2009). Not only Flo proteins, but several other yeast adhesins such as Als and Epa proteins from C. albicans and C. glabrata respectively, contain tandem repeat (TR) regions. So far, their structures are unknown, but recently ab initio modeling with either Rosetta or LINUS generated consistent structures of three-stranded anti-parallel β-sheet domains for the TR regions of Als5p (Fig. 3c) (Frank et al. 2010).
Searching the pool of all known yeast adhesins using the TANGO program (http://tango.crg.es/), many adhesins were found to contain sequences with high β-aggregation potential, including Flo1p and Flo11p (Ramsook et al. 2009). When a peptide from the central domain of Flo1p (aa 305–315) was suspended in neutral buffer, it readily formed amyloids. It was proposed that amyloid formation plays a role in cell adhesion by increasing the avidity of the adhesins by “bundling” the adhesins. Therefore, amyloid formation can greatly increase the intercellular binding strength by increasing avidity (Otoo et al. 2008; Frank et al. 2010; Ramsook et al. 2010).
Glycosylation of Flo proteins
Flo proteins are heavily O- and N-glycosylated (Straver et al. 1994a; Bony et al. 1997). Glycosylation is an enzyme-directed and site-specific process, which is required for proper functioning of the protein. It is a post-translational modification and the protein is glycosylated on its way through the endoplasmic reticulum and the Golgi apparatus. N-glycosylation is the addition of oligosaccharides to the amide group of asparagines and results in highly branched and extended high mannose structures (Dean 1999). O-glycosylation occurs at a later stage during protein processing and short linear chains up to five mannose residues are added to the β-hydroxyl group of serine and threonine residues (Tanner and Lehle 1987; Aikawa 1995; Goto 2007). Proteins of diverse yeast species contain other O- and N-linked oligosaccharide structures (glycans). The differences found in various yeast species, has been reviewed (Gemmill and Trimble 1999). Sugar analysis of a homolog of Flo1p revealed the presence of 64% carbohydrate, in the molar ratio of 60:27:1, which corresponds to mannose, glucose and N-acetylglucosamine respectively. However, the exact glycan profile of cell wall Flo proteins is not yet known (Straver et al. 1994b; Bony et al. 1997; Douglas et al. 2007).
The role of direct carbohydrate-carbohydrate interactions has not yet been explored in yeast flocculation. Recently, it was shown that these interactions can be important for cell adhesion phenomena since glycans occur on the outermost cell periphery and therefore are likely involved in the first intercellular contact (Bucior and Burger 2004; Bucior et al. 2009). Carbohydrate chains offer a rich supply of potential low-affinity binding sites, arranged in a polyvalent array that may create a flexible and versatile carbohydrate-carbohydrate recognition system (Misevic and Burger 1986). This then allows cells to explore surrounding surfaces, and to form or reinforce interactions before subsequent steps in the cell that are mediated by tight and stable covalent bonds (Mallinson et al. 2003). The interaction between individual carbohydrates is fairly weak, and biologically relevant high affinities are achieved by the organisation of cell surface proteoglycans, glycoproteins or glycolipids into clusters or superstructures (Hakomori 2003; Yoneda and Couchman 2003; Todeschini and Hakomori 2008). These provide avidities that can attach cells to each other under physiological conditions. Carbohydrate self-recognition takes place through surfaces determined by carbohydrate epitopes and is based on non-covalent bonds: van der Waals contacts, hydrogen bonds, electrostatic forces and interactions with cations (Spillmann and Burger 1996).
Carbohydrate self-interactions have been recently well described for sponge proteoglycans. Dissociated sponge cells from two different species have the capacity to recognise specifically their own species and reaggregate through cell-surface proteoglycans, termed aggregation factors (AFs) (Fernàndez-Busquets and Burger 2003). In a calcium-independent process the AFs adhere to the cell surface, and in a calcium-dependent process they exhibit AF self-association (Jarchow et al. 2000; Haseley et al. 2001; Fernàndez-Busquets and Burger 2003; Carvalho de Souza et al. 2009). AFs are large molecules (2 × 104 to 2 × 107 Da) that are composed of 30–60% carbohydrates. Atomic Force Microscope (AFM) visualisation has revealed either a linear or a sunburst-like core structure with 20–25 radiating arms (Dammer et al. 1995). Single-molecule force spectroscopy measurements reported equally strong adhesion forces between glycan molecules (190–310 pN) as between proteins in antibody-antigen interactions (244 pN) (Bucior et al. 2004). This observation suggested the existence of intermolecular carbohydrate adhesion domains (Garcia-Manyes et al. 2006).
Cell–cell adhesion biophysics
Molecular and genetic approaches have identified various cell adhesion molecules (CAMs) with their ligand specificities, and have determined the processes in which they are involved. However, the molecular mechanisms by which CAMs regulate different types of adhesion are open debates (Morgan et al. 2007; Ludwig et al. 2008). To understand cell adhesion, the vast amount of qualitative data that is available must be augmented with quantitative data of the biophysics of adhesion. Historically, the strength of cell adhesion to a substrate has been studied using simple washing assays (Klebe 1974). Washing assays have proven to be versatile and useful in identifying CAMs, important extra-cellular-matrix components and other proteins that are involved in various forms of cell adhesion. To estimate the force to which cells are subjected, various assays that are based on the regulated flow of media have been implemented, including flow chamber methods (Kaplanski et al. 1993). However, these assays only give estimates of adhesion forces, since the shear force that is exerted on the cells depends on parameters such as cell size, cell shape and how the cell is attached to the substrate. Recently, single-cell and single-molecule techniques have been developed to obtain more controlled and quantitative measurements of adhesion strength.
Single-cell force spectroscopy assays on living cells have been applied to measure the strength of cell adhesion down to single-molecule levels (Helenius et al. 2008). A living cell can be attached to a tipless cantilever of an AFM and the interacting partner (molecule or cell) on a substrate-coated surface. Alternatively, the living cell can be fixed on a surface and the tip functionalised with the interacting molecule. AFM force spectroscopy with a single cantilever-bound cell can be used to investigate cell–cell and cell matrix interactions. The approach and withdrawal of this cell to and from its surface can be precisely controlled by parameters such as applied force, contact time and pulling speed by benefiting from the AFM’s high-force sensitivity and spatial resolution. The data collected in these experiments include information on repulsive forces before contact, cell deformability, maximum unbinding forces, individual unbinding events, and the total work required to remove a cell from the surface (Table 2). Force spectroscopy can identify cell subpopulations and characterise the regulation of cell adhesion events with single-molecule resolution (Taubenberger et al. 2007). Analysis of discrete cell adhesion forces demonstrated a dynamic increase of adhesion over time.
Single S. cerevisiae cells have been attached to a tipless AFM cantilever, and used as a living single-cell probe to perform single-cell force spectroscopy (Kang and Elimelech 2009). The contributions of several galectin family members in cell-substratum adhesion of Madin-Darby canine kidney cells have been studied using quantitative single cell AFM force spectroscopy (Friedrichs et al. 2007) (Table 2). Optical tweezers have been used to orient uropathogenic Escherichia coli (which present a FimH lectin at the tip of their type 1 pilus) relative to a mannose-presenting surface, and thus, limit the number of points of attachment (Liang et al. 2000) (Table 2). It was possible to quantify the forces required to break a single interaction between pilus and mannose groups.
Carbohydrate–lectin interactions studied by single-cell (SCFS) and single-molecule (SMFS) force spectroscopy
SCFS rupture force (pN)
SMFS rupture force (pN)
Surface-expressed mannose residues
Baumgart and Offenhausser (2003)
Hanley et al. (2004)
Hanley et al. (2004)
PMN and LS174T
Red blood cells
Helix pomatia lectin
Saccharides from blood types O and A
Grandbois et al. (2000)
Gad et al. (1997)
117 ± 41
Touhami et al. (2003b)
121 ± 53
Touhami et al. (2003b)
Madin-Darby canine kidney cells
Collagen-I coated substrate
Friedrichs et al. (2007)
Madin-Darby canine kidney cells
Galectin-3 and -9
Laminin-111 coated substrate
Friedrichs et al. (2007)
Carbohydrate-lectin unbinding forces measured using single-molecule AFM force spectroscopy
Component attached to AFM probe
Unbinding force (pN)
(AB)2 agglutinin (Ricinus communis)
Lactose (p-amino-phenyl lactoside)
Agglutinin bound to Sepharose 4B beads
58 ± 9
Dettmann et al. (2000)
(AB)2 agglutinin (Viscum album)
Lactose (p-amino- phenyl lactoside)
Agglutinin bound to Sepharose 4B beads
47 ± 7
Dettmann et al. (2000)
ConAa (Jack bean)
Con A on gold surface
96 ± 55
Touhami et al. (2003a)
Con A (jack bean)
Mannose bound to SAMsd on gold-coated silicon
47 ± 41
Ratto et al. (2004)
FimH (E. coli)
FimH on polysty-rene plates
Galectin-1 (bovine heart)
Lactose (p-amino-phenyl lactoside)
Galectin-1 bound to Sepharose 4B beads
34 ± 6
Dettmann et al. (2000)
Galectin-derivatised glass surface
Gunning et al. (2009)
Medical and industrial significance of S. cerevisiae flocculation phenotype
In hazardous environmental conditions, S. cerevisiae cells possess the remarkable properties to adhere to other cells or to substrates like agar or plastics. Adhesion to surfaces is a mechanism that may lead to biofilm formation. It is often used as a model to study biofilm formation of pathogenic yeasts, responsible for 41% of the mortality rate in hospitals (Wisplinghoff et al. 2004). Biofilms give protection to yeast cells, e.g. by conferring resistance to antifungal drugs. Adhesion to abiotic surfaces as catheters or prostheses can serve as a reservoir of pathogenic cells ready to gain access to the bloodstream of patients (Jabra-Rizk et al. 2001, 2004; Donlan and Costerton 2002; Kojic and Darouiche 2004). A detailed study of this mechanism is necessary in the search for an adequate antifungal treatment.
The flocculation phenomenon is exploited in the brewery industry as an easy, convenient and cost-effective way to separate the aggregated yeast cells from the beer at the end of the primary fermentation. The timing of flocculation is crucial for brewers as the quality of beer highly depends on it. When cells start to flocculate too early, the fermentation will be incomplete with undesirable aromas and too many residual sugars. On the other hand, when the flocculation is delayed, problems can arise during beer filtration (Willaert 2007a). A good understanding of the underlying mechanism and the factors affecting flocculation is of crucial importance for the performance and control of the brewing process (Verstrepen et al. 2003). Another application is the self-aggregation of yeast cells, which can be classified as a type of cell immobilisation (Willaert and Baron 1996, Willaert 2007b). Flocculent yeast strains can be selected to obtain a high cell density during the beer fermentation process (Nedovic et al. 2005; Willaert and Nedovic 2006; Verbelen et al. 2010). Consequently, the volumetric productivity can be increased considerably.
Altogether, elucidating the structure and the physical binding properties of adhesins will allow controlling yeast adhesion more efficiently in medical and biotechnological applications.
This work is supported by the Belgian Federal Science Policy Office (Belspo) and European Space Agency (ESA) PRODEX program, the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen), and the Research Council of the Vrije Universiteit Brussel.