Biotechnology Letters

, Volume 32, Issue 5, pp 649–654

In vitro release behavior and cytotoxicity of doxorubicin-loaded gold nanoparticles in cancerous cells

Original Research Paper

DOI: 10.1007/s10529-010-0208-x

Cite this article as:
Asadishad, B., Vossoughi, M. & Alamzadeh, I. Biotechnol Lett (2010) 32: 649. doi:10.1007/s10529-010-0208-x

Abstract

Doxorubicin (DOX), a common cancer chemotherapeutics, was conjugated to folate-modified thiolated-polyethylene glycol-functionalized gold nanoparticles. The in vitro, controlled release behavior of DOX-loaded gold nanoparticles was observed using porous dialysis membranes (cut-off = 2 kDa). DOX-loaded gold nanoparticles had higher cytotoxicity for folate-receptor-positive cells (KB cells) compared to folate-receptor-negative cells (A549 cells) which were 48 and 62% viable for 10 μM doxorubicin, respectively. This indicates the potential of these nano-carriers for targeted-delivery. In addition, healthy cell viability was 69% for 10 μM free doxorubicin whereas for the same content of drug in DOX-loaded nanoparticles healthy cell viability increased to 80%.

Keywords

Cancerous cellsCytotoxicityControlled releaseGold nanoparticlesIn vitroThiolated-polyethylene glycol

Supplementary material

10529_2010_208_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 50 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Chemical and Petroleum EngineeringSharif University of TechnologyTehranIran
  2. 2.Institute of NanoscienceSharif University of TechnologyTehranIran
  3. 3.Biochemical and Bioenvironmental Research CenterSharif University of TechnologyTehranIran