Original Research Paper

Biotechnology Letters

, Volume 31, Issue 8, pp 1217-1222

First online:

Comparison of methods to assess the enzyme accessibility and hydrolysis of pretreated lignocellulosic substrates

  • Richard P. ChandraAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia
  • , Shannon M. EwanickAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia
  • , Pablo A. ChungAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia
  • , Kathy Au-YeungAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia
  • , Luis Del RioAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia
  • , Warren MabeeAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia
  • , Jack N. SaddlerAffiliated withDepartment of Wood Science, Faculty of Forestry, University of British Columbia Email author 

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Abstract

Fiber size analysis, water retention value, and Simons’ stain measurements were assessed for their potential to predict the susceptibility of a given substrate to enzymatic hydrolysis. Slight modifications were made to the fiber size analysis and water retention protocols to adapt these measurements to evaluate substrates for cellulolytic hydrolysis rather than pulps for papermaking. Lodgepole pine was pretreated by the steam and ethanol-organosolv processes under varying conditions. The Simons’ stain procedure proved to be an effective method for indicating the potential ease of enzymatic hydrolysis of substrates pretreated by either process or when the pretreatment conditions were altered.

Keywords

Bioconversion Cellulase Ethanol Simons’ stain