, Volume 31, Issue 5, pp 653-658,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 25 Jan 2009

Quantitative identification of teratoma tissues formed by human embryonic stem cells with TeratomEye

Abstract

An automated vision system, TeratomEye, was developed for the identification of three representative tissue types: muscle, gut and neural epithelia which are commonly found in teratomas formed from human embryonic stem cells. Muscle tissue, a common structure was identified with an accuracy of 90.3% with high specificity and sensitivity greater than 90%. Gut epithelia were identified with an accuracy of 87.5% with specificity and sensitivity greater than 80%. Neural epithelia which were the most difficult structures to distinguish gave an accuracy of 47.6%. TeratomEye is therefore useful for the automated identification of differentiated tissues in teratoma sections.