Biotechnology Letters

, 32:143

Diastereoselective synthesis of l-threo-3,4-dihydroxyphenylserine by low-specific l-threonine aldolase mutants

Original Research Paper

DOI: 10.1007/s10529-009-0125-z

Cite this article as:
Gwon, HJ. & Baik, SH. Biotechnol Lett (2010) 32: 143. doi:10.1007/s10529-009-0125-z

Abstract

Diastereoselectivity-enhanced mutants of l-threonine aldolase (l-TA) for l-threo-3,4-dihydroxyphenylserine (l-threo-DOPS) synthesis were isolated by error-prone PCR followed by a high-throughput screening. The most improved mutant was achieved from the mutant T3-3mm2, showing a 4-fold increase over the wild-type l-TA. When aldol condensation activity was examined using whole cells of T3-3mm2, its de was constantly maintained at 55% during the batch reactions for 80 h, yielding 3.8 mg l-threo-DOPS/ml.

Keywords

Diastereoselectivity l-threo-3,4-Dihydroxyphenylserine l-Threonine aldolase 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Advanced Radiation Technology InstituteKorea Atomic Energy Research InstituteJeonbukRepublic of Korea
  2. 2.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science TechnologyDaejonKorea
  3. 3.Department of Food Science and Human Nutrition, and Research Institute of Human EcologyChonbuk National UniversityJeonbukKorea