Biotechnology Letters

, 31:1775

Stress responses to heterologous membrane protein expression in Escherichia coli

Original Research Paper

DOI: 10.1007/s10529-009-0075-5

Cite this article as:
Xu, L.Y. & James Link, A. Biotechnol Lett (2009) 31: 1775. doi:10.1007/s10529-009-0075-5

Abstract

The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.

Keywords

G protein-coupled receptorsHeterologous protein expressionSigma factorStress response

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Departments of Chemical EngineeringPrinceton UniversityPrincetonUSA
  2. 2.Departments of Molecular BiologyPrinceton UniversityPrincetonUSA