Biotechnology Letters

, Volume 30, Issue 12, pp 2097–2103

Re-engineering Escherichia coli for ethanol production

  • L. P. Yomano
  • S. W. York
  • S. Zhou
  • K. T. Shanmugam
  • L. O. Ingram
Original Research Paper

DOI: 10.1007/s10529-008-9821-3

Cite this article as:
Yomano, L.P., York, S.W., Zhou, S. et al. Biotechnol Lett (2008) 30: 2097. doi:10.1007/s10529-008-9821-3

Abstract

A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and adhB) into the chromosome. By selecting for fermentative growth in mineral salts medium containing xylose, a highly productive strain was isolated in which the ethanol cassette had been integrated behind the rrlE promoter, designated strain LY160 (KO11, Δfrd::celYEc ΔadhE ΔldhA, ΔackAlacA::casABKorrlE::(pdcZm-adhAZm-adhBZm-FRT-rrlE) pflB+). This strain fermented 9% (w/v) xylose to 4% (w/v) ethanol in 48 h in mineral salts medium, nearly equal to the performance of KO11 with Luria broth.

Keywords

BetaineE. coliEthanolLactateOsmotic stress

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • L. P. Yomano
    • 1
  • S. W. York
    • 1
  • S. Zhou
    • 1
  • K. T. Shanmugam
    • 1
  • L. O. Ingram
    • 1
  1. 1.Department Microbiology and Cell ScienceUniversity of FloridaGainesvilleUSA