Original Research Paper

Biotechnology Letters

, Volume 30, Issue 10, pp 1705-1711

First online:

Cloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibody

  • Wooseok ShinAffiliated withDepartment of Genetic Engineering, Sungkyunkwan University
  • , Byungwoo LeeAffiliated withDepartment of Genetic Engineering, Sungkyunkwan University
  • , Sungyoul HongAffiliated withDepartment of Genetic Engineering, Sungkyunkwan University
  • , Chongsuk RyouAffiliated withSanders Brown Center on Aging, University of KentuckyDepartment of Microbiology, Immunology and Molecular Genetics, College of Medicine, University of Kentucky
  • , Moosik KwonAffiliated withDepartment of Genetic Engineering, Sungkyunkwan University Email author 

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Abstract

A PrP gene, from a Korean bovine, exhibiting a nonsense and a missense polymorphism respectively at nucleotides 576 and 652 has been cloned. The latter resulted in Glu to Lys substitution at amino acid residue 218. After expression and purification of the recombinant bovine PrP (recBoPrP) with Glu218Lys substitution, a polyclonal antibody against this protein was raised. ELISA and Western blot analysis suggested that the recBoPrP obtained in this study had a unique conformation not presented in native PrPC, and the polyclonal antibody recognized PrP in a conformation dependent manner. These reagents will be valuable tools for studying PrP conformation.

Keywords

Anti-bovine prion protein antibody Korean bovine Prion protein Prion protein gene