Biotechnology Letters

, Volume 30, Issue 7, pp 1163–1168

Bone morphogenetic protein-2 enhances bone regeneration mediated by transplantation of osteogenically undifferentiated bone marrow-derived mesenchymal stem cells

Original Research Paper

DOI: 10.1007/s10529-008-9675-8

Cite this article as:
Kang, SW., La, WG., Kang, J.M. et al. Biotechnol Lett (2008) 30: 1163. doi:10.1007/s10529-008-9675-8


We have hypothesized that human bone marrow-derived mesenchymal stem cells (BMMSCs), that are not osteogenically differentiated prior to implantation, would regenerate bone extensively in vivo once exogenous bone morphogenetic protein-2 (BMP-2) was delivered to the implantation site. BMP-2 released from heparin-conjugated poly(lactic-co-glycolic acid) (HCPLGA) scaffolds stimulates osteogenic differentiation of cultured BMMSCs. Upon implantation, undifferentiated BMMSCs on BMP-2-loaded HCPLGA scaffolds induce far more extensive bone formation than either undifferentiated BMMSCs or osteogenically differentiated BMMSCs on HCPLGA scaffolds. These BMP-2-loaded HCPLGA scaffolds could prove invaluable for in vivo regeneration of bone from undifferentiated human BMMSCs.


Bone marrow-derived stem cellBone morphogenetic protein-2Bone regenerationTissue engineering

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Chemical EngineeringHanyang UniversitySeoulKorea
  2. 2.Department of BioengineeringHanyang UniversitySeoulKorea
  3. 3.Department of Orthopaedic Surgery, Ansan Hospital, College of MedicineKorea UniversityAnsanKorea