, Volume 30, Issue 5, pp 791-799

Metabolic flux analysis in biotechnology processes

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Abstract

Metabolic flux analysis (MFA) has become a fundamental tool of metabolic engineering to elucidate the metabolic state of the cell and has been applied to various biotechnological processes. In recent years, considerable technical advances have been made. Developments of analytical instruments allow us to determine 13C labeling distribution of intracellular metabolites with high accuracy and sensitivity. Moreover, kinetic information of intracellular label distribution during isotopic instationary enables us to calculate metabolic fluxes with shortened experimental time and decreased amount of labeled substrate. The 13C MFA may be one of the most promising approaches for the target estimation to improve strain performances and production processes.