Original Research Paper

Biotechnology Letters

, Volume 30, Issue 5, pp 853-860

First online:

Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli

  • Mahbuba RahmanAffiliated withDepartment of Bioscience and Bioinformatics, Faculty of Computer Science and System Engineering, Kyushu Institute of Technology
  • , Mohammad Rubayet HasanAffiliated withDepartment of Bioscience and Bioinformatics, Faculty of Computer Science and System Engineering, Kyushu Institute of Technology
  • , Kazuyuki ShimizuAffiliated withDepartment of Bioscience and Bioinformatics, Faculty of Computer Science and System Engineering, Kyushu Institute of TechnologyInstitute for Advanced Biosciences, Keio University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (∼1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.

Keywords

Batch fermentation Escherichia coli Global regulators Metabolic pathways rpoS