Biotechnology Letters

, Volume 30, Issue 5, pp 853–860

Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli

Authors

  • Mahbuba Rahman
    • Department of Bioscience and Bioinformatics, Faculty of Computer Science and System EngineeringKyushu Institute of Technology
  • Mohammad Rubayet Hasan
    • Department of Bioscience and Bioinformatics, Faculty of Computer Science and System EngineeringKyushu Institute of Technology
    • Department of Bioscience and Bioinformatics, Faculty of Computer Science and System EngineeringKyushu Institute of Technology
    • Institute for Advanced BiosciencesKeio University
Original Research Paper

DOI: 10.1007/s10529-007-9621-1

Cite this article as:
Rahman, M., Hasan, M.R. & Shimizu, K. Biotechnol Lett (2008) 30: 853. doi:10.1007/s10529-007-9621-1

Abstract

Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (∼1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.

Keywords

Batch fermentationEscherichia coliGlobal regulatorsMetabolic pathwaysrpoS

Copyright information

© Springer Science+Business Media B.V. 2008