Biotechnology Letters

, Volume 30, Issue 4, pp 731–734

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Original Research Paper

DOI: 10.1007/s10529-007-9599-8

Cite this article as:
Ji, XJ., Huang, H., Li, S. et al. Biotechnol Lett (2008) 30: 731. doi:10.1007/s10529-007-9599-8


Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.


2,3-Butanediol Klebsiella oxytoca Proton-suicide method 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Xiao-Jun Ji
    • 1
  • He Huang
    • 1
  • Shuang Li
    • 1
  • Jun Du
    • 1
  • Min Lian
    • 1
  1. 1.College of Life Science and PharmacyNanjing University of TechnologyNanjingP.R. China

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