Biotechnology Letters

, Volume 30, Issue 3, pp 405–410

Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration

  • Chang Seok Ki
  • Sook Young Park
  • Hyun Jeong Kim
  • Hong-Moon Jung
  • Kyung Mi Woo
  • Jung Weon Lee
  • Young Hwan Park
Original Research Paper

DOI: 10.1007/s10529-007-9581-5

Cite this article as:
Ki, C.S., Park, S.Y., Kim, H.J. et al. Biotechnol Lett (2008) 30: 405. doi:10.1007/s10529-007-9581-5
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Abstract

We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.

Keywords

BiomaterialsCell adhesionCell proliferationFibroinsOsteoblasts

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Chang Seok Ki
    • 1
    • 2
  • Sook Young Park
    • 2
    • 3
  • Hyun Jeong Kim
    • 2
    • 3
  • Hong-Moon Jung
    • 4
  • Kyung Mi Woo
    • 4
  • Jung Weon Lee
    • 5
  • Young Hwan Park
    • 1
    • 2
  1. 1.Department of Biosystems and Biomaterials Science and EngineeringSeoul National UniversitySeoulRepublic of Korea
  2. 2.Intelligent Textile Research CenterSeoul National UniversitySeoulRepublic of Korea
  3. 3.Department of Dental Anesthesiology and Dental Research Institute, School of DentistrySeoul National UniversitySeoulRepublic of Korea
  4. 4.Department of Cell & Developmental Biology, School of DentistrySeoul National UniversitySeoulRepublic of Korea
  5. 5.Cancer Research Institute, Department of Tumor Biology, College of MedicineSeoul National UniversitySeoulRepublic of Korea