Biotechnology Letters

, Volume 30, Issue 3, pp 547–554

Transgenic cotton expressing synthesized scorpion insect toxin AaHIT gene confers enhanced resistance to cotton bollworm (Heliothis armigera) larvae

  • Jiahe Wu
  • Xiaoli Luo
  • Zhian Wang
  • Yingchuan Tian
  • Aihua Liang
  • Yi Sun
Original Research Paper

DOI: 10.1007/s10529-007-9555-7

Cite this article as:
Wu, J., Luo, X., Wang, Z. et al. Biotechnol Lett (2008) 30: 547. doi:10.1007/s10529-007-9555-7

Abstract

A synthetic scorpion Hector Insect Toxin (AaHIT) gene, under the control of a CaMV35S promoter, was cloned into cotton via Agrobacterium tumefaciens-mediated transformation. Southern blot analyses indicated that integration of the transgene varied from one to more than three estimated copies per genome; seven homozygous transgenic lines with one copy of the T-DNA insert were then selected by PCR and Southern blot analysis. AaHIT expression was from 0.02 to 0.43% of total soluble protein determined by western blot. These homozygous transgenic lines killed larvae of cotton bollworm (Heliothis armigera) by 44–98%. The AaHIT gene could used therefore an alternative to Bt toxin and proteinase inhibitor genes for producing transgenic cotton crops with effective control of bollworm.

Keywords

Androctonus australisBollworm controlCotton (transgenic)Gossypium hirsutumScorpion toxin in cotton

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jiahe Wu
    • 1
    • 2
  • Xiaoli Luo
    • 1
  • Zhian Wang
    • 1
  • Yingchuan Tian
    • 2
  • Aihua Liang
    • 1
  • Yi Sun
    • 1
  1. 1.Institute of Cotton Research and Biotechnology Research CentreShanxi Academy of Agricultural SciencesTaiyuanP.R. China
  2. 2.National Key Laboratory of Plant Genomics, Institute of MicrobiologyChinese Academy of SciencesBeijingP.R. China