Biotechnology Letters

, Volume 30, Issue 2, pp 197–203

Osteogenic differentiation effects on rat bone marrow-derived mesenchymal stromal cells by lentivirus-mediated co-transfection of human BMP2 gene and VEGF165 gene

Original Research Paper

DOI: 10.1007/s10529-007-9532-1

Cite this article as:
Jiang, J., Fan, C. & Zeng, B. Biotechnol Lett (2008) 30: 197. doi:10.1007/s10529-007-9532-1

Abstract

We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were successfully co-expressed in MSCs confirmed by real-time PCR and ELISA. And the alkaline phosphatase activity of MSCs was significantly augmented by the co-transfection with both genes than any single gene transfection (P < 0.01). These results demonstrated the feasibility of the combined gene therapy by using MSCs lentivirally co-transfected with hVEGF165 and hBMP2 for bone regeneration.

Keywords

Bone morphogenetic protein 2Co-transfectionLentiviral vectorOsteogenic differentiationVascular endothelial growth factor

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Orthopedics, The Sixth Affiliated People’s HospitalShanghai Jiao Tong UniversityShanghaiChina