Abstract
Porous fiber-reinforced chitosan nerve conduits were fabricated from chitosan yarns and a chitosan solution by combining an industrial braiding method with a mold casting/lyophilization technique. The conduits were permeable to molecules ranging in molecular size from 180 Da (glucose) to 66,200 Da (BSA). The compressive load of the reinforced conduits was significantly higher than that of a non-reinforced control conduit at equal levels of strain. The tensile strength of the reinforced conduits was also increased from 0.41 ± 0.17 to 3.69 ± 0.64 MPa. An in vitro cytotoxicity test showed the conduits were not cytotoxic to Neuro-2a cells. Preliminary in vivo implantation testing indicated that the conduits were compatible with the surrounding tissue.
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References
Aebischer P, Guenard V, Valentini RF (1990) The morphology of regenerating peripheral nerves is modulated by the surface microgeometry of polymeric guidance channels. Brain Res 531:211–218
Gong HP, Zhong YH, Li JC et al (2000) Studies on nerve cell affinity of chitosan-derived materials. J Biomed Mater Res 52:285–295
Jenq CB, Coggeshall RE (1987) Permeable tubes increase the length of the gap that regenerating axons can span. Brain Res 408:239–242
Madihally SV, Matthew HW (1999) Porous chitosan scaffolds for tissue engineering. Biomaterials 20:1133–1142
Patel M, Vandevord PJ, Matthew H et al (2006) Video-gait analysis of functional recovery of nerve repaired with chitosan nerve guides. Tissue Eng 12:3189–3199
Rodriguez FJ, Gomez N, Perego G et al (1999) Highly permeable polylactide-caprolactone nerve guides enhance peripheral nerve regeneration through long gaps. Biomaterials 20:1489–1500
Wang AJ, Ao Q, Cao WL et al (2005a) Fiber-based chitosan tubular scaffolds for soft tissue engineering: fabrication and in vitro evaluation. Tsinghua Sci Technol 10:449–453
Wang X, Hu W, Cao Y et al (2005b) Dog sciatic nerve regeneration across a 30-mm defect bridged by a chitosan/PGA artificial nerve graft. Brain 128:1897–1910
Wang AJ, Ao Q, He Q et al (2006a) Neural stem cell affinity of chitosan and feasibility of chitosan-based porous conduits as scaffolds for nerve tissue engineering. Tsinghua Sci Technol 11:415–420
Wang AJ, Cao WL, Gong K et al (2006b) Development of porous chitosan tubular scaffolds for tissue engineering applications. Asian Chitin J 2:53–60
Yang YM, Gu XS, Tan RX et al (2004) Fabrication and properties of a porous chitin/chitosan conduit for nerve regeneration. Biotechnol Lett 26:1793–1797
Acknowledgments
The authors thank Mr. Bryan Brown for his helpful advice. This work was financially supported by National Basic Research Program of China (also called “973” Program, No. 2005CB623905), the Tsinghua-Yue-Yuen Medical Science Fund, Beijing Municipal Science & Technology Commission (No. H060920050430) and the National Natural Science Foundation of China (No. 30670528).
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Aijun Wang and Qiang Ao contributed equally to this work.
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Wang, A., Ao, Q., Wei, Y. et al. Physical properties and biocompatibility of a porous chitosan-based fiber-reinforced conduit for nerve regeneration. Biotechnol Lett 29, 1697–1702 (2007). https://doi.org/10.1007/s10529-007-9460-0
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DOI: https://doi.org/10.1007/s10529-007-9460-0