Phytase expression in transgenic soybeans: stable transformation with a vector-less construct
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- Gao, X.R., Wang, G.K., Su, Q. et al. Biotechnol Lett (2007) 29: 1781. doi:10.1007/s10529-007-9439-x
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A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T1 plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T2 progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T3 transgenic seeds as compared to 64 FTU/kg in wild-type plants.