Biotechnology Letters

, Volume 29, Issue 11, pp 1781–1787

Phytase expression in transgenic soybeans: stable transformation with a vector-less construct

  • Xiao Rong Gao
  • Guo Kun Wang
  • Qiao Su
  • Yan Wang
  • Li Jia An
Original Research Paper

DOI: 10.1007/s10529-007-9439-x

Cite this article as:
Gao, X.R., Wang, G.K., Su, Q. et al. Biotechnol Lett (2007) 29: 1781. doi:10.1007/s10529-007-9439-x

Abstract

A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T1 plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T2 progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T3 transgenic seeds as compared to 64 FTU/kg in wild-type plants.

Keywords

Pollen tube pathway transformationLinear DNAPhytase geneTransgenic soybean

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Xiao Rong Gao
    • 1
  • Guo Kun Wang
    • 1
  • Qiao Su
    • 1
  • Yan Wang
    • 1
  • Li Jia An
    • 1
  1. 1.Department of Bioscience and BiotechnologyDalian University of TechnologyDalianP.R. China