Biotechnology Letters

, Volume 29, Issue 10, pp 1605–1610

Expression of a ribosome-inactivating protein gene in bitter melon is induced by Sphaerotheca fuliginea and abiotic stimuli

Authors

  • Jun Xu
    • State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
    • Graduate School of the Chinese Academy of Sciences
  • Hualin Wang
    • State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences
    • The Laboratory of Toxicology, College of Public HealthZheng Zhou University
Original Research Paper

DOI: 10.1007/s10529-007-9433-3

Cite this article as:
Xu, J., Wang, H. & Fan, J. Biotechnol Lett (2007) 29: 1605. doi:10.1007/s10529-007-9433-3

Abstract

The gene encoding a single-chain, ribosome-inactivating protein (SCRIP) was cloned from bitter melon (Momordica charantia L.) leaves infected with the fungus, Sphaerotheca fuliginea, by RT-PCR. The ORF was 861 bp. The ribosome-inactivating protein was expressed in E. coli and, when purified, it inhibited the growth of the Sphaerotheca fuliginea in vitro. Northern blot analysis revealed that RIP transcripts rapidly accumulated in leaves 1-day post inoculation with Sphaerotheca fuliginea and reached a peak at 3 d. The expression pattern of RIP induced by methyl jasmonate and salicylic acid were different from that of pathogen-induced expression. Mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of RIP transcripts. Abscisic acid also induced transcription of RIPs. The signal compounds, ethylene and okadaic acid, induced a moderate accumulation of RIP transcripts.

Keywords

Bitter melonFungal infectionMomordica charantiaRibosome-inactivating protein Sphaerotheca

Copyright information

© Springer Science+Business Media B.V. 2007