Biotechnology Letters

, Volume 29, Issue 10, pp 1459–1464

Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells

  • Xiaoxiang Li
  • Xiaoli Wang
  • Shaoling Xiong
  • Jing Zhang
  • Litao Cai
  • Yanyan Yang
Original Research Paper

DOI: 10.1007/s10529-007-9426-2

Cite this article as:
Li, X., Wang, X., Xiong, S. et al. Biotechnol Lett (2007) 29: 1459. doi:10.1007/s10529-007-9426-2

Abstract

A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using Ni2+ resins. The recombinant NK, ∼30 kDa, retained fibrinolytic activity (60 U/ml). The integration of the EGFP expression cassette in the Bac-to-Bac system is thus an effective method for the expression and purification of recombinant NK protein in Spodoptera frugiperda insect cells.

Keywords

Bac-to-Bac Expression Nattokinase Purification Spodoptera frugiperda 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Xiaoxiang Li
    • 1
  • Xiaoli Wang
    • 1
  • Shaoling Xiong
    • 1
  • Jing Zhang
    • 1
  • Litao Cai
    • 1
  • Yanyan Yang
    • 1
  1. 1.College of Life SciencesHubei UniversityWuhanChina