Biotechnology Letters

, Volume 29, Issue 3, pp 439–445

Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3

  • S. Anil Kumar
  • Majid Kazemian Abyaneh
  • S. W. Gosavi
  • Sulabha K. Kulkarni
  • Renu Pasricha
  • Absar Ahmad
  • M. I. Khan
Original Research Paper

DOI: 10.1007/s10529-006-9256-7

Cite this article as:
Anil Kumar, S., Abyaneh, M.K., Gosavi, S.W. et al. Biotechnol Lett (2007) 29: 439. doi:10.1007/s10529-006-9256-7

Abstract

Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

Keywords

Capping peptideEnzymatic methodNitrate reductaseSilver nanoparticles

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • S. Anil Kumar
    • 1
  • Majid Kazemian Abyaneh
    • 2
  • S. W. Gosavi
    • 2
  • Sulabha K. Kulkarni
    • 2
  • Renu Pasricha
    • 3
  • Absar Ahmad
    • 1
  • M. I. Khan
    • 1
  1. 1.Biochemical Sciences DivisionNational Chemical LaboratoryPuneIndia
  2. 2.DST Unit on Nanoscience, Department of PhysicsPune UniversityPuneIndia
  3. 3.Materials Chemistry DivisionNational Chemical LaboratoryPuneIndia