Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3
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- Anil Kumar, S., Abyaneh, M.K., Gosavi, S.W. et al. Biotechnol Lett (2007) 29: 439. doi:10.1007/s10529-006-9256-7
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Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10–25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.