Original Research Paper

Biotechnology Letters

, Volume 28, Issue 24, pp 2033-2038

First online:

Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae

  • Qing-Xue KongAffiliated withSchool of Chemical Engineering and Technology, Tianjin UniversityDepartment of Food Science, Tianjin Agricultural College
  • , Ji-Guang GuAffiliated withCollege of Life Science and Technology, Jinan University
  • , Li-Min CaoAffiliated withSchool of Chemical Engineering and Technology, Tianjin University
  • , Ai-Li ZhangAffiliated withSchool of Chemical Engineering and Technology, Tianjin University
  • , Xun ChenAffiliated withSchool of Chemical Engineering and Technology, Tianjin University
  • , Xue-Ming ZhaoAffiliated withSchool of Chemical Engineering and Technology, Tianjin University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

To improve ethanol production in Saccharomyces cerevisiae, two yeast strains were constructed. In the mutant KAM-3, the FPS1 gene, which encodes a channel protein responsible for glycerol export, was deleted. The mutant KAM-11 had the GLT1 gene (encoding glutamate synthase) placed under the PGK1 promoter while having the FPS1 deletion. Growth rate and biomass concentration remained virtually unchanged with the mutant KAM-11, compared to that of the parent. Over-expression of GLT1 by the PGK1 promoter along with FPS1 deletion resulted in a 14% higher ethanol production and a 30% lower glycerol formation compared to the parental strain under anaerobic fermentation conditions. Furthermore, acetate and pyruvic acid formation was also reduced in order for cells to maintain redox balance.

Keywords

Channel protein gene Ethanol Glutamate synthase gene Glycerol Saccharomyces cerevisiae