Biotechnology Letters

, Volume 28, Issue 23, pp 1909–1912

Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.

Original Research Paper

DOI: 10.1007/s10529-006-9175-7

Cite this article as:
Han, SQ., Ouyang, PK., Wei, P. et al. Biotechnol Lett (2006) 28: 1909. doi:10.1007/s10529-006-9175-7


The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52–100% and 88–99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.


BiotransformationCommon vetchCyanohydrinOxynitrilaseVicia sativa L.

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Shi-Qing Han
    • 1
  • Ping-Kai Ouyang
    • 1
  • Ping Wei
    • 1
  • Yi Hu
    • 1
  1. 1.College of Pharmacy and Life ScienceNanjing University of TechnologyNanjingChina