Cultivation of Microorganisms in the Cultural Medium Made from Squid Internal Organs and Accumulation of Polyunsaturated Fatty Acids in the Cells
Received: 04 May 2005 Accepted: 05 May 2005 DOI:
Cite this article as: Morita, N., Ichise, N., Yumoto, I. et al. Biotechnol Lett (2005) 27: 933. doi:10.1007/s10529-005-7187-3 Abstract
The disposal and more efficient utilization of marine wastes is becoming increasingly serious. A culture media for microorganisms has been prepared from squid internal organs that are rich in polyunsaturated fatty acids (PUFAs). Both freshwater and marine bacteria grew well in this medium and some bacteria accumulated PUFAs in their lipids, suggesting uptake of exogenous PUFAs. Higher PUFA accumulations were observed in
Escherichia coli mutant cells defective either in unsaturated fatty acid biosynthesis or fatty acid degradation, or both, compared to those without these mutations. Therefore, PUFA accumulation in cells can be improved by genetic modification of fatty acid metabolism in the bacteria. Squid internal organs would be a good source of medium, not only for marine bacteria but also for freshwater bacteria, and that this process may be advantageous to make efficient use of the fishery wastes and to produce PUFA-containing microbial cells and lipids. Keywords docosahexaenoic acid (DHA) efficient utilization of fishery wastes eicosapentaenoic acid (EPA) squid organ culture medium polyunsaturated fatty acids References Ando, S, Nakajima, K, Hatano, M 1992 Incorporation of n-3 polyunsaturated fatty acids into phospholipids of a bacterium Vibrio sp. cultivated with sardine oil J. Ferment. Bioeng 73 169 171 CrossRef Aoki, H, Miyamoto, N, Furuya, Y, Mankura, M, Endo, Y, Fujimoto, K 2002 Incorporation and accumulation of docosahexaenoic acid from the medium by Pichia methanolica HA-32 Biosci. Biotechnol. Biochem 66 2632 2638 CrossRef PubMed Bligh, EG, Dyer, WJ 1959 A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol 37 911 917 PubMed Brachmann, CB, Davies, A, Cost, GJ, Caputo, E, Li, J, Hieter, P, Boeke, JD 1998 Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications Yeast 14 115 132 CrossRef PubMed Exterkate, FA, Otten, BJ, Wassenberg, HW, Veerkamp, JH 1971 Comparison of the phospholipid composition of Bifidobacterium and Lactobacillus strains J. Bacteriol 106 824 829 PubMed Fang, J, Kato, C, Sato, T, Chan, O, McKay, D 2004 Biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria Comp. Biochem. Physiol. B Biochem. Mol. Biol 137 455 461 CrossRef PubMed Farrell, DJ 1998 Enrichment of hen eggs with n-3 long-chain fatty acids and evaluation of enriched eggs in humans Am. J. Clin. Nutr 68 538 544 PubMed Hayashi, K 1996 Composition and distribution of lipids in different tissues of the arrow squid Loligo bleekeri Fisheries Sci 62 84 87 Hayashi, M, Yukino, T, Maruyama, I, kido, S, Kitano, S 2001 Uptake and accumulation of exogenous docosahexaenoic acid by Chlorella Biosci. Biotechnol. Biochem 65 202 204 CrossRef PubMed
Hossain Z, Konishi M, Hosokawa M, Takahashi K, (2005) Effect of polyunsaturated fatty acid-enriched phosphatidylcholine and phosphatidylserine on butyrate-induced growth inhibition, differentiation and apoptosis in Caco-2 cells.
Cell Biochem. Funct. In press Kanai, K, Kondo, E 1979 Antibacterial and cytotoxic aspects of long-chain fatty acids as cell surface events: selected topics Jpn. J. Med. Sci. Biol 32 135 174 PubMed Morita, N, Nishida, T, Tanaka, M, Yano, Y, Okuyama, H 2005 Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria Biotechnol. Lett 27 389 393 CrossRef PubMed Papanikolaou, S, Aggelis, G 2003 Selective uptake of fatty acids by the yeast Yarrowia lipolytica Eur. J. lipid Sci. Technol 105 651 655 CrossRef Rizzo, AF, Korkeala, H, Mononen, I 1987 Gas chromatography analysis of cellular fatty acids and neutral monosaccharides in the identification of lactobacilli Appl. Environ. Microbiol 53 2883 2888 PubMed Saito, H, Tomioka, H, Yoneyama, T 1984 Growth of group IV mycobacteria on medium containing various saturated and unsaturated fatty acids Antimicrob. Agents Chemother 26 164 169 PubMed Satomi, M, Oikawa, H, Yano, Y 2003 Shewanella marinintestina sp. Nov., Shewanella schlegeliana sp. Nov. and Shewanella sairae sp. Nov., novel eicosapentaenoic acid-producing marine bacteria isolated from sea-animal intestines Int. J. Syst. Evol. Microbiol 53 491 499 CrossRef PubMed Valentine, RC, Valentine, DL 2004 Omega-3 fatty acids in cellular membranes: a unified concept Prog. Lipid Res 43 383 402 CrossRef PubMed Watanabe, K, Ishikawa, C, Inoue, H, Cenhua, D, Yazawa, K, Kondo, K 1994 Incorporation of exogenous docosahexaenoic acid into various bacterial phospholipids J. Am. Oil Chem. Soc 71 325 330 Yumoto, I, Iwata, H, Sawabe, T, Ueno, K, Ichise, N, Matsuyama, H, Okuyama, H, Kawasaki, K 1999 Characterization of a facultatively psychrophilic bacterium, Vibrio rumoiensis sp. Nov., that exhibits high catalase activity Appl. Environ. Microbiol 65 67 72 PubMed