Biotechnology Letters

, Volume 27, Issue 12, pp 817–822

Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis

Article

DOI: 10.1007/s10529-005-6717-3

Cite this article as:
Kim, GB., Brochet, M. & Lee, B.H. Biotechnol Lett (2005) 27: 817. doi:10.1007/s10529-005-6717-3

Abstract

A gene coding for bile salt hydrolase (BSH) from Bifidobacterium adolescentis was cloned and expressed in Escherichia coli, and the nucleotide sequence was determined. The BSH of E. coli transformants was produced intracellularly in the absence of bile salts. A unique bsh promoter (Pbsh) sequence was identified by using a Neural Network Promoter Prediction (NNPP, version 2.2). In spite of their high-level sequence homology with other bsh genes in the Bifidobacterium species, their genetic organization surrounding the bsh gene and their promoter sequences are different depending on the species.

Keywords

Bifidobacteriumadolescentisbile salt hydrolase (BSH)bsh promotergene cloningsequence homology

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Department of Food Science and Agricultural ChemistryMcGill UniversityQuebecCanada
  2. 2.Food Research and Development CenterAgriculture and Agri-Food CanadaQuebecCanada
  3. 3.Unité de génomique des microorganismes pathogènesInstitut PasteurCedex 15France