Biotechnology Letters

, Volume 28, Issue 6, pp 425–430

Polycyclic Aromatic Hydrocarbon (PAH) Degradation Coupled to Methanogenesis

Authors

    • Department of Chemical EngineeringUniversity of Maryland
  • Youngsoon Um
    • Department of Chemical EngineeringUniversity of Maryland
  • Tracey R. Pulliam Holoman
    • Department of Chemical EngineeringUniversity of Maryland
Article

DOI: 10.1007/s10529-005-6073-3

Cite this article as:
Chang, W., Um, Y. & Holoman, T.R.P. Biotechnol Lett (2006) 28: 425. doi:10.1007/s10529-005-6073-3

Abstract

Baltimore Harbor (Baltimore, MD) sediments were utilized to initiate anaerobic enrichment cultures with polycyclic aromatic hydrocarbons (PAHs) in the absence of supplementary electron acceptors. Cultures amended with naphthalene and phenanthrene exhibited sustained, transferable degradation of the PAHs. Bromoethanesulfonic acid, a selective inhibitor of methanogenesis, inhibited the degradation of 200 μm naphthalene and phenanthrene; molecular characterization based on 16S rRNA sequences confirmed that methanogenic Archaea were eliminated, thus providing evidence that methanogenesis is involved in the degradation pathway.

Keywords

16S ribosomal RNAanaerobic biodegradationbromoethanesulfonic acid (BES)methanogenesispolycyclic aromatic hydrocarbon (PAH)

Copyright information

© Springer 2006