Biotechnology Letters

, Volume 28, Issue 4, pp 223–231

The Tre2 Oncoprotein, Implicated in Ewing’s Sarcoma, Interacts with Two Components of the Cytoskeleton

  • Christophe Dechamps
  • Stephane Bach
  • Daniel Portetelle
  • Micheline Vandenbol
Article

DOI: 10.1007/s10529-005-5523-2

Cite this article as:
Dechamps, C., Bach, S., Portetelle, D. et al. Biotechnol Lett (2006) 28: 223. doi:10.1007/s10529-005-5523-2

Abstract

The product of the Tre2 oncogene, structurally related to the Ypt/RabGTPase-activating proteins (Ypt/RabGAP), is involved in various human cancers, including Ewing’s sarcoma. In order to identify proteins interacting with the GAP part of this protein, we performed yeast two-hybrid screening of two libraries. Two components of the cytoskeleton were thus identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments. The proteins found to interact with the GAP region are the light regulatory chain of myosin II (Myl2) and LOC91256, a protein containing ankyrin repeats.

Keywords

interactionLOC91526Myl2oncogeneTre2/Tre17/USP6

Copyright information

© Springer 2006

Authors and Affiliations

  • Christophe Dechamps
    • 1
  • Stephane Bach
    • 2
  • Daniel Portetelle
    • 1
  • Micheline Vandenbol
    • 1
  1. 1.Animal and Microbial Biology UnitGembloux Agricultural UniversityGemblouxBelgium
  2. 2.C.N.R.S. UPS2682 “Molecules and Therapeutic Targets”Roscoff cedex, BretagneFrance