Biotechnology Letters

, Volume 27, Issue 22, pp 1829–1832

Production of Vanillin by Metabolically Engineered Escherichia coli

  • Sang-Hwal Yoon
  • Cui Li
  • Ju-Eun Kim
  • Sook-Hee Lee
  • Ji-Young Yoon
  • Myung-Suk Choi
  • Weon-Taek Seo
  • Jae-Kyung Yang
  • Jae-Yeon Kim
  • Seon-Won Kim
Article

DOI: 10.1007/s10529-005-3561-4

Cite this article as:
Yoon, S., Li, C., Kim, J. et al. Biotechnol Lett (2005) 27: 1829. doi:10.1007/s10529-005-3561-4

Abstract

E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l−1 was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.

Keywords

ferulic acidmetabolic engineeringrecombinant E. colivanillin

Copyright information

© Springer 2005

Authors and Affiliations

  • Sang-Hwal Yoon
    • 1
  • Cui Li
    • 1
  • Ju-Eun Kim
    • 2
  • Sook-Hee Lee
    • 2
  • Ji-Young Yoon
    • 1
  • Myung-Suk Choi
    • 3
  • Weon-Taek Seo
    • 4
  • Jae-Kyung Yang
    • 3
  • Jae-Yeon Kim
    • 2
    • 5
  • Seon-Won Kim
    • 1
    • 2
    • 5
  1. 1.Department of Food Science & NutritionGyeongsang National UniversityJinjuKorea
  2. 2.Division of Applied Life Science (BK21)Gyeongsang National UniversityJinjuKorea
  3. 3.Division of Forest ScienceGyeongsang National UniversityJinjuKorea
  4. 4.Department of Food ScienceJinju National UniversityJinjuKorea
  5. 5.Environmental Biotechnology National Core Research CenterGyeongsang National UniversityJinjuRepublic of Korea