Biotechnology Letters

, Volume 27, Issue 6, pp 389–393

Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria

Authors

    • Research Institute of Genome-based BiofactoryNational Institute of Advanced Industrial Science and Technology (AIST)
  • Takanori Nishida
    • Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth ScienceHokkaido University
  • Mika Tanaka
    • Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth ScienceHokkaido University
  • Yutaka Yano
    • National Research Institute of Fisheries Science
  • Hidetoshi Okuyama
    • Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth ScienceHokkaido University
Article

DOI: 10.1007/s10529-005-1532-4

Cite this article as:
Morita, N., Nishida, T., Tanaka, M. et al. Biotechnol Lett (2005) 27: 389. doi:10.1007/s10529-005-1532-4

Abstract

When docosahexaenoic acid (DHA)-producing Moritella marina strain MP-1 was cultured in the medium containing 0.5 μ g cerulenin ml−1, an inhibitor for fatty acid biosynthesis, the cells grew normally, but the␣content of DHA in the total fatty acids increased from 5.9–19.4%. The DHA yield of M. marina strain MP-1 cells also increased from 4 to 13.7 mg l−1 by cerulenin treatment. The same effect of cerulenin was observed in eicosapentaenoic acid (EPA)-producing Shewanellamarinintestina strain IK-1 grown in the medium containing 7.5 μg cerulenin ml−1, and the cerulenin treatment increased the EPA yield from 1.6 to 8 mg l−1. The use of cerulenin is, therefore, advantageous to increase the content of intracellular polyunsaturated fatty acids (PUFA) in particular PUFA-containing phospholipids in bacterial cells.

Keywords

cerulenindocosahexaenoic acid (DHA)eicosapentaenoic acid (EPA)polyunsaturated fatty acid (PUFA)PUFA-producing bacteria

Copyright information

© Springer 2005