Biotechnology Letters

, Volume 27, Issue 4, pp 279–282

Nitrogen-dependent regulation of medium-chain length polyhydroxyalkanoate biosynthesis genes in pseudomonads


  • Nils Hoffmann
    • Institute of Molecular BiosciencesMassey University
    • Institute of Molecular BiosciencesMassey University

DOI: 10.1007/s10529-004-8353-8

Cite this article as:
Hoffmann, N. & Rehm, B.H.A. Biotechnol Lett (2005) 27: 279. doi:10.1007/s10529-004-8353-8


Comparative transcriptional analysis of polyhdroxyalkanoate (PHA) biosynthesis genes with wild type strains and mutants, which lack the intact alternative sigma factor gene rpoN, was performed using semi-quantitative RT-PCR. In Pseudomonas putida and Pseudomonas aeruginosa,phaI and phaF were co- transcribed. PhaF was a negative regulator of transcription of PHA synthase gene phaC1 but did not serve as auto-repressor. However, the alternative sigma factor RpoN is suggested as negative regulator of phaF transcription. In P. putida, phaI-phaF transcription is strongly dependent on nitrogen availability and PHA accumulation, whereas phaF transcription is not. These data suggested a differential regulation of phaF and phaIF. The phaC1 gene transcription occurred almost independently by of RpoN or nitrogen availability in both pseudomonads.



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© Springer 2005