Bone loss induced by ovariectomy in rats is prevented by gene transfer of parathyroid hormone or an Arg-Gly-Asp-containing peptide Article Received: 05 November 2004 Accepted: 11 November 2004 DOI:
Cite this article as: Chen, W., Liu, J., Diao, W. et al. Biotechnol Lett (2005) 27: 41. doi:10.1007/s10529-004-6577-2 Abstract
Osteoporosis is a major and growing healthcare concern as the population ages. The genes of both a Arg-Gly-Asp (RGD)-containing peptide and parathyroid hormone (PTH) were used to reduce bone loss induced by ovariectomy (OVX) in rats. Plasmids with either RGD or PTH gene were delivered into the quadriceps of OVX rats. The expression of the genes was detected by RT-PCR and radioimmunoassay. Analysis of bone mineral density, bone mechanical testing and bone mineral content indicated an improvement in bone properties in both RGD-transferred and PTH-transferred rats compared to OVX rats. Gene transfer of either RGD or PTH is therefore a possible approach to prevent bone loss in OVX rats thus providing a potential method to prevent osteoporosis in clinical situations.
Keywords electroporational gene transfer osteoporosis ovariectomized rats parathyroid hormone RGD-peptide
These authors contributed equally to this work
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