Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins
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Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.
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- Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins
Volume 26, Issue 22 , pp 1731-1737
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- Kluwer Academic Publishers-Plenum Publishers
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- flow cytometry
- Real Time RT-PCR
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- Author Affiliations
- 1. School of Biotechnological Sciences, University of Naples “Federico II”, Via Università, 133, Portici, Naples, 80055, Italy
- 2. Food Science Department, University of Naples “Federico II”, Via Università, 100, Portici, Naples, 80055, Italy