Biochemical Genetics

, Volume 47, Issue 1, pp 8–18

Relationships Among Genetic Makeup, Active Ingredient Content, and Place of Origin of the Medicinal Plant Gastrodia Tuber

Authors

    • School of Biology and Food EngineeringChangsha University of Science and Technology
    • Molecular Biology Research Center, School of Biological Science and TechnologyCentral South University
  • Zhi-yong Luo
    • Molecular Biology Research Center, School of Biological Science and TechnologyCentral South University
  • Chikira Ismail Msangi
    • Molecular Biology Research Center, School of Biological Science and TechnologyCentral South University
  • Xiao-shun Shu
    • School of Biology and Food EngineeringChangsha University of Science and Technology
  • Li Wen
    • School of Biology and Food EngineeringChangsha University of Science and Technology
  • Shui-ping Liu
    • Molecular Biology Research Center, School of Biological Science and TechnologyCentral South University
  • Chang-quan Zhou
    • Hunan De-hai Corporation for Pharmacy
  • Rui-xin Liu
    • School of Biology and Food EngineeringChangsha University of Science and Technology
    • Molecular Biology Research Center, School of Biological Science and TechnologyCentral South University
Article

DOI: 10.1007/s10528-008-9201-7

Cite this article as:
Tao, J., Luo, Z., Msangi, C.I. et al. Biochem Genet (2009) 47: 8. doi:10.1007/s10528-008-9201-7

Abstract

Gastrodia tuber and its component gastrodin have many pharmacological effects. The chemical fingerprints and gastrodin contents of eight Gastrodia populations were determined, and the genomic DNA polymorphism of the populations was investigated. Genetic distance coefficients among the populations were calculated using the DNA polymorphism data. A dendrogram of the genetic similarities between the populations was constructed using the genetic distance coefficients. The results indicated that the genomic DNA of Gastrodia tubers was highly polymorphic; the eight populations clustered into three major groups, and the gastrodin content varied greatly among these groups. There were obvious correlations among genetic makeup, gastrodin content, and place of origin. The ecological environments in Guizhou and Shanxi may be conducive to evolution and to gastrodin biosynthesis, and more suitable for cultivation of Gastrodia tubers. These findings may provide a scientific basis for overall genetic resource management and for the selection of locations for cultivating Gastrodia tubers.

Keywords

DNA polymorphismGastrodia elata BlumeGastrodinGenetic relationshipsChemical fingerprints

Supplementary material

10528_2008_9201_MOESM1_ESM.doc (778 kb)
MOESM1 Genomic DNA amplification products from eight Gastrodia tuber samples, using (A) primer 6; (B) primer 10; (C) primer 12; (D) primer 14; and (E) primer 15. (DOC 777 kb)

Copyright information

© Springer Science+Business Media, LLC 2008