Allozyme Variability and Phylogenetic Relationships in Honey Bee (Hymenoptera: Apidae: Apis mellifera) Populations From Greece and Cyprus
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Ten gene enzymic systems (α-GPDH, AO, MDH, ADH, LAP, SOD, ALP, ACPH, ME, and EST), corresponding to 12 genetic loci, were assayed from five Greek populations representing three subspecies of Apis mellifera, A. m. cecropia (Pthiotida, Kythira), A. m. macedonica (Macedonia), and the “Aegean race” of A. mellifera, which is supposed to be very similar to A. m. adami (Ikaria, Kasos), as well as a population from Cypus (A. m. cypria). ADH ∗-1, ADH ∗-2, and LAP ∗ electrophoretic patterns discriminate the Cyprus population from the Greek populations. MDH ∗-1, EST ∗-3, SOD ∗, ALP ∗, and ME ∗ loci were found to be polymorphic in almost all populations. The observed heterozygosity was found to range from 0.066 to 0.251. Allele frequencies of all loci were used to estimate Nei's genetic distance, which was found to range between 0.011 and 0.413 among the populations studied. UPGMA and neighbor-joining phylogenetic trees obtained by genetic distance matrix methods, as well as a Wagner tree based on the discrete character parsimony method, support the hypothesis that the most distant population is that from Cyprus. Our allozymic data support A. m. cypria as a distinct subspecies, but there was no allozymic support for the distinction of the other subspecies existing in Greece.
- Alahiotis, S. N., and Berger, E. (1977). Isozyme and allozyme patterns in embryonic Drosophila cell culture lines. Biochem. Genet. 15:877–883. CrossRef
- Ashton, G. C., and Braden, A. W. H. (1961). Serum β-globulin polymorphism in mice. Austr. J. Exp. Biol. Med. Sci. 14:228.
- Avise, J. C. (1994). Molecular Markers, Natural History and Evolution, Chapman and Hall, New York.
- Ayala, F. J., Powell, J. R.,. Tracey, M. L., Mourao, C. A., and Perez-Salas, S. (1972). Enzyme variability in the Drosophila willistoni group IV. Genic variation in natural populations of Drosophila willistoni. Genetics 70:113–139.
- Badino, G., Celebrano, G., Manino, A., and Ifantidis, M. D. (1988). Allozyme variability in Greek honeybees (Apis mellifera L.). Apidologie 19(4):377–386.
- Berlocher, S. H. (1980). An electrophoretic key for distinguishing species of the genus Rhagoletis (Diptera: Tephritidae) as larvae, pupae, or adults. Ann. Entomol. Soc. Am. 73:131–137.
- Bernatchez, L., and Osinov, A. (1995). Genetic diversity of trout (genus Salmo) from its most eastern native range based on mitochondrial DNA and nuclear gene variation. Mol. Ecol. 4:285–297.
- Bouga, M., Harizanis, P. C., Kilias, G., and Alahiotis, S. (2005). Genetic divergence and phylogenetic relationships of honey bee A. mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR-RFLP analysis of three mtDNA segments. Apidologie 36:335–344. CrossRef
- Buth, D. G., and Murphy, R. W. (1999). The use of isozyme characters in systematic studies. Biochem. Syst. Ecol. 27:117–129.
- Cornuet, J. M. (1979). The MDH system in honeybees of Guadeloup. J. Hered. 70:223–224.
- Daly, H. V. (1991). Systematics and identification of Africanized honey bees, In Spivak, M., Fletcher, D. J. C., and Breed, M. D. (eds.), The “African” Honey Bee, Westview Press, Boulder, Colorado, pp. 13–44.
- Dedej, S., Basiolo, A., and Piva, R. (1996). Morphometric and alloenzymatic characterisation in the Albanian honeybee population Apis mellifera L. Apidologie 27(3):121–131.
- Estoup, A., Garnery, L., Solignac, M., and Cornuet, J. M. (1995). Microsatellite variation in honey bee (Apis mellifera L.) populations: Hierarchical genetic structure and test of the infinite allele and stepwise mutation models. Genetics 140:679–695.
- Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39(4):783–791.
- Felsenstein, J. (1993). PHYLIP (Phylogeny Inference Package), Version 3.5C. Distributed by the author, Department of Genetics, University of Washington, Seattle.
- Ferguson, M. M., Danzmann, R. G., and Hutchings, J. A. (1991). Incongruent estimates of population differentiation among brook charr, Salvelinus fontinalis, from Cape Race, Newfoundland, Canada, based upon allozyme and mitochondrial DNA variation. J. Fish Biol. 39:79–85.
- Ifantidis, M. D. (1979). Morphological characters of the Greek honeybee Apis mellifica cecropia. Proceedings of the 27th Apimondia International Apicultural Congress, Athens, pp. 285–294.
- Kandemir, I., Meixner, M. D., and Sheppard, W. S. (2003). Morphometric, allozymic, and mtDNA variation in honeybee (Apis mellifera cypria, Pollman 1879) populations in northern Cyprus. Final Program and Book of Abstracts, 38th Apimondia International Apicultural Congress, p. 798.
- Kluge, A. G., and Farris, J. S. (1969). Quantitive phyletics and the evolution of anurans. Syst. Zool. 18:1–32.
- Mantel, N. A. (1967). The detection of disease clustering and a generalized regression approach. Cancer Res. 27:209–220.
- Moritz, C., Dowling, T. E., and Brown, W. M. (1987). Evolution of animal mitochondrial DNA: Relevance for population biology and systematics. Ann. Rev. Ecol. Syst. 18:269–292. CrossRef
- Nei, M. (1972). Genetic distance between populations. Am. Naturalist 106:283–291. CrossRef
- Nunamaker, R. A., Wilson, W. T., and Haley, B. E. (1984). Electrophoretic detection of Africanized honey bee (Apis mellifera scutellata) in Guatemala and Mexico based on malate dehydrogenase allozyme patterns. J. Kans. Entomol. Soc. 57:622–631.
- Page, R. D. M. (1996). TreeView: An application to display phylogenetic trees on personal computers. Comp. Appl. Biosci. 12:357–358.
- Papasotiropoulos, V., Klossa-Kilia, E., Kilias, G., and Alahiotis, S. (2001). Genetic divergence and phylogenetic relationships in grey mullets (Teleostei: Mugilidae) using allozyme data. Biochem. Genet. 39:155–168. CrossRef
- Poulik, M. D. (1957). Starch gel electrophoresis in a discontinuous system of buffers. Nature 180:1477–1479.
- Rice, W. R. (1989). Analyzing tables of statistical tests. Evolution 43(1):223–225.
- Richardson, B. J., Baverstock, P. R., and Adams, M. (1986). Allozyme Electrophoresis. Academic, New York.
- Robinson, G. E., and Page, R. E. Jr. (1988). Genetic determination of guarding and undertaking in honey bee colonies. Nature 333:356–358. CrossRef
- Robinson, G. E., and Page, R. E. Jr. (1989). Genetic determination of nectar foraging, pollen foraging, and nest-site scouting in honey bee colonies. Behav. Ecol. Sociobiol. 24:317–323. CrossRef
- Robinson, G. E., Page, R. E. Jr., and Fondrk, M. K. (1990). Intracolonial behavioural variation in worker oviposition, oophagy, and larval care in queenless honey bee colonies. Behav. Ecol. Sociobiol. 26:315–323. CrossRef
- Rohlf, J. (1990). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, Exeter Software, New York.
- Ruttner, F. (1988). Biogeography and Taxonomy of Honeybees, Springer–Verlag, Berlin.
- Ruttner, F. (1992). Naturgeschichte der Honigbienen, Ehrenwirth Verlag, Munich, Germany.
- Saitou, N., and Nei, M. (1987). The neighbour-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4):406–425.
- Shaw, C. R., and Prasad, R. (1970). Starch gel electrophoresis of enzymes: A compilation of recipes. Biochem. Genet. 4:297–320.
- Schiff, N. M., and Sheppard, W. S. (1995). Genetic analysis of commercial honey bees (Hymenopetera, Apidae) from the southeastern United States. J. Econ. Entomol. 88(5):1216–1220.
- Sheppard, W. S. (1988). Comparative study of enzyme polymorphism in United States and European honey bee (Hymenoptera: Apidae) populations. Ann. Entomol. Soc. Am. 81:886–889.
- Sheppard, W. S., and Berlocher, S. H. (1989). Allozyme variation and differentiation among four Apis species. Apidologie 20(5):419–431.
- Sheppard, W. S., and Huettel, M. D. (1988). Biochemical genetic markers, intraspecific variation, and population genetics of the honey bee. In Needham, G. R., Page Jr., R. E., Delfinado-Baker, M., and Bowman, C. E. (eds.), Africanized Honey Bees and Bee Mites, Ellis-Horwood, Chichester, England, pp. 281–286.
- Sheppard, W. S., and McPheron, B. A. (1986). Genetic variation in honeybees from an area of racial hybridization in western Czechoslovakia. Apidologie 17(1):21–23.
- Sheppard, W. S., and Meixner, M. D. (2003). Apis mellifera pomonella, a new honey bee subspecies from Central Asia. Apidologie 34:367–375. CrossRef
- Sheppard, W. S., and Smith, D. R. (2000). Identification of African-derived bees in the Americas: A survey of methods. Ann. Entomol. Soc. Am. 93:159–176.
- Sheppard, W. S., Arias, M. C., Greech, A., and Meixner, M. D. (1997). Apis mellifera ruttneri, a new honey bee subspecies from Malta. Apidologie 28:287–293.
- Sneath, P. H. A., and Sokal, R. R. (1973). Numerical Taxonomy: The principle and practice of numerical classification. W. H. Freeman, San Francisco.
- Sylvester, H. A. (1982). Electrophoretic identification of Africanized honeybees. J. Apic. Res. 21 (2):93–97.
- Sylvester, H. A. (1986). Biochemical Genetics. In Rinderer, T. E. (ed.), Bee Genetics and Breeding, Academic Press, Orlando, FL, pp. 177–203.
- Swofford, D. L., and Selander, R. B. (1981). BIOSYS-1: A computer program for the analysis of allelic variation in genetics. Rel. 1.0. Department of Genetics and Development, University of Illinois, Urbana-Champaign.
- Visscher, P. K. (1996). Reproductive conflict in honey bees: A stalemate of worker egg-laying and policing. Behav. Ecol. Sociobiol. 39:237–244. CrossRef
- Wright, S. (1965). The interpretation of population structure by F-Statistics with special regard to systems of mating. Evolution 19:395–420.
- Allozyme Variability and Phylogenetic Relationships in Honey Bee (Hymenoptera: Apidae: Apis mellifera) Populations From Greece and Cyprus
Volume 43, Issue 9-10 , pp 471-483
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- Apis mellifera
- genetic structure
- phylogenetic relationships
- Author Affiliations
- 1. Department of Biology, Division of Genetics, Cell Biology and Development, University of Patras, Rio, 265 00, Patras, Greece
- 2. Laboratory of Sericulture-Apiculture, Agricultural University of Athens, 75 Iera Odos 118 55, Athens, Greece