Biochemical Genetics

, Volume 43, Issue 3, pp 165–173

Molecular Cloning and Characterization of a Novel Human C4orf13 Gene, Tentatively a Member of the Sodium Bile Acid Cotransporter Family

  • Xianqiong Zou
  • Dianzuo Wang
  • Guanzhou Qiu
  • Chaoneng Ji
  • Feng Jin
  • Maoqing Wu
  • Huarui Zheng
  • Xin Li
  • Liyun Sun
  • Yu Wang
  • Rong Tang
  • Robert Chunhua Zhao
  • Yumin Mao
Article

DOI: 10.1007/s10528-005-1509-y

Cite this article as:
Zou, X., Wang, D., Qiu, G. et al. Biochem Genet (2005) 43: 165. doi:10.1007/s10528-005-1509-y

Abstract

By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human cDNA (C4orf13). This cDNA is 2706 bp in length, encoding a 340-amino-acid polypeptide that contains a typical SBF (sodium bile acid cotransporter family) domain and ten possible transmembrane segments. The putative protein C4orf13 shows high similarity with its orthologs in Mus musculus and Xenopus laevis. Human C4orf13 is mapped to chromosome 4q31.2 and contains 12 exons. RT-PCR analysis shows that human C4orf13 is widely expressed in human tissues, and the expression levels in liver and lung are relatively high, expression levels in placenta, kidney, spleen, and thymus are moderate, low levels of expression are detected in heart, prostate, and testis.

Keywords:

sodium bile acid cotransporter family human C4orf13 expression RT-PCR 

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Xianqiong Zou
    • 1
    • 2
  • Dianzuo Wang
    • 1
  • Guanzhou Qiu
    • 1
  • Chaoneng Ji
    • 2
  • Feng Jin
    • 2
  • Maoqing Wu
    • 2
  • Huarui Zheng
    • 2
  • Xin Li
    • 2
  • Liyun Sun
    • 2
  • Yu Wang
    • 2
  • Rong Tang
    • 2
  • Robert Chunhua Zhao
    • 3
  • Yumin Mao
    • 2
  1. 1.School of Resource Processing and Bio-engineeringCentral South UniversityChangshaP.R. China
  2. 2.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life ScienceFudan UniversityShanghaiP.R. China
  3. 3.State Key Laboratory of Experimental HematologyChinese Academy of Medical Science and Peking Union Medical CollegeBeijingP.R. China