BioControl

, Volume 53, Issue 4, pp 667–683

The role of phytohormones in basal resistance and Trichoderma-induced systemic resistance to Botrytis cinerea in Arabidopsis thaliana

Article

DOI: 10.1007/s10526-007-9103-3

Cite this article as:
Korolev, N., Rav David, D. & Elad, Y. BioControl (2008) 53: 667. doi:10.1007/s10526-007-9103-3

Abstract

Thirty-six phytohormone-affected mutants of Arabidopsis thaliana (L.) Heynh. and their parental ecotypes were tested for resistance/susceptibility to Botrytis cinerea Pers.; Fr. and ability to develop Trichoderma-mediated induced systemic resistance (ISR). Ecotype Colombia-0 (Col-0) was relatively resistant to B. cinerea, and Trichoderma harzianum Rifai T39 application at sites spatially separated (roots) from the B. cinerea inoculation (leaves) resulted in reduction of grey mold symptoms. Ecotypes Wassilewskija-4, Nossen-0 and Landsberg-0 had low levels of basal resistance to B. cinerea and were unable to express ISR. Mutants derived from ISR-non-inducible ecotypes displayed ISR-non-inducible phenotypes, whereas the ISR inducibility of mutants derived from the ISR-inducible genotype Col-0 varied according to the type of mutant. Thus, salicylic acid (SA)-impaired mutants derived from Col-0 were ISR-inducible, while ethylene/jasmonic acid (ethylene/JA)-impaired mutants of the same origin were ISR-non-inducible. SA-impaired mutants retained basal level of resistance to B. cinerea, while most ethylene/JA-impaired mutants were highly susceptible. Abscisic acid- and gibberellin-impaired mutants were highly susceptible to B. cinerea and showed ISR-non-inducible phenotypes irrespective of their lines of origin. Auxin-resistant mutants derived from Col-0 were ISR-inducible; mutant originating from Landsberg-0 and mutants which were resistant to both auxin and ethylene were ISR-non-inducible. Most of the arabidopsis genotypes which were unable to express Trichoderma-mediated ISR against B. cinerea exhibited enhanced susceptibility to this pathogen. T. harzianum treatments enhanced the growth of arabidopsis plants regardless of genotype or ISR inducibility.

Keywords

Abscisic acidArabidopsis thalianaAuxinBotrytis cinereaEthyleneGibberellinInduced systemic resistanceJasmonic acidSalicylic acidTrichoderma harzianum

Abbreviations

ABA

Abscisic acid

GA

Gibberellic acid

HR

Hypersensitive response

IAA

Indole-3-acetic acid

ISR

Induced systemic resistance

JA

Jasmonic acid

SA

Salicylic acid

SAR

Systemic acquired resistance

PR

Pathogenesis-related

PGPR

Plant growth promoting rhizobacteria

Copyright information

© International Organization for Biological Control (IOBC) 2007

Authors and Affiliations

  1. 1.Department of Plant Pathology and Weed ResearchARO, The Volcani CenterBet DaganIsrael