Biogerontology

, Volume 15, Issue 2, pp 165–176

Cell proliferation arrest and redox state status as part of different stages during senescence establishment in mouse fibroblasts

  • Francisco Triana-Martínez
  • Norma E. López-Diazguerrero
  • Luis A. Maciel-Barón
  • Sandra L. Morales-Rosales
  • Sonia Galván-Arzate
  • Francisco J. Fernandez-Perrino
  • Alejandro Zentella
  • Viviana I. Pérez
  • Luis E. Gomez-Quiroz
  • Mina Königsberg
Research Article

DOI: 10.1007/s10522-013-9488-6

Cite this article as:
Triana-Martínez, F., López-Diazguerrero, N.E., Maciel-Barón, L.A. et al. Biogerontology (2014) 15: 165. doi:10.1007/s10522-013-9488-6

Abstract

Senescence phenotype can be achieved by multiple pathways. Most of them involve the activation of negative cell cycle regulators as well as a shift to an oxidative status. However, the exact participation of these events in senescence establishment and maintenance is not completely understood. In this study we investigated the content of three final cell cycle regulators, as well as the redox state in some critical points during the pre-senescent and the full-senescent states. Our results highlight the existence of a critical pre-phase in senescent phenotype establishment, in which cell proliferation stops with the participation of the cell cycle inhibitors, and a second maintenance stage where the exacerbated pro-oxidant state inside the cell induces the physiological decline characteristic in senescent cells.

Keywords

Senescence P16 P21 P27 Oxidative stress Cell cycle 

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Francisco Triana-Martínez
    • 1
    • 2
  • Norma E. López-Diazguerrero
    • 1
  • Luis A. Maciel-Barón
    • 1
    • 2
  • Sandra L. Morales-Rosales
    • 1
  • Sonia Galván-Arzate
    • 3
  • Francisco J. Fernandez-Perrino
    • 4
  • Alejandro Zentella
    • 5
    • 6
  • Viviana I. Pérez
    • 7
  • Luis E. Gomez-Quiroz
    • 1
  • Mina Königsberg
    • 1
  1. 1.Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la SaludUniversidad Autónoma Metropolitana-IztapalapaMexicoMexico
  2. 2.Posgrado en Biologia ExperimentalMexico CityMexico
  3. 3.Dept. NeuroquímicaInstituto Nacional de Neurología y NeurocirugíaMexicoMexico
  4. 4.Dept. Biotecnología, DCBSUniversidad Autónoma Metropolitana IztapalapaMexicoMexico
  5. 5.Dept. Medicina Genómica y Toxicología Ambiental. IIBUNAMMexicoMexico
  6. 6.Depto BioquímicaINCMNSZMexicoMexico
  7. 7.Department of Biochemistry & BiophysicsOregon State UniversityCorvallisUSA

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