Research Article

Biogerontology

, Volume 15, Issue 2, pp 165-176

First online:

Cell proliferation arrest and redox state status as part of different stages during senescence establishment in mouse fibroblasts

  • Francisco Triana-MartínezAffiliated withDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-IztapalapaPosgrado en Biologia Experimental
  • , Norma E. López-DiazguerreroAffiliated withDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa
  • , Luis A. Maciel-BarónAffiliated withDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-IztapalapaPosgrado en Biologia Experimental
  • , Sandra L. Morales-RosalesAffiliated withDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa
  • , Sonia Galván-ArzateAffiliated withDept. Neuroquímica, Instituto Nacional de Neurología y Neurocirugía
  • , Francisco J. Fernandez-PerrinoAffiliated withDept. Biotecnología, DCBS, Universidad Autónoma Metropolitana Iztapalapa
  • , Alejandro ZentellaAffiliated withDept. Medicina Genómica y Toxicología Ambiental. IIB, UNAMDepto Bioquímica, INCMNSZ
  • , Viviana I. PérezAffiliated withDepartment of Biochemistry & Biophysics, Oregon State University
  • , Luis E. Gomez-QuirozAffiliated withDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa
    • , Mina KönigsbergAffiliated withDepartamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa Email author 

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Abstract

Senescence phenotype can be achieved by multiple pathways. Most of them involve the activation of negative cell cycle regulators as well as a shift to an oxidative status. However, the exact participation of these events in senescence establishment and maintenance is not completely understood. In this study we investigated the content of three final cell cycle regulators, as well as the redox state in some critical points during the pre-senescent and the full-senescent states. Our results highlight the existence of a critical pre-phase in senescent phenotype establishment, in which cell proliferation stops with the participation of the cell cycle inhibitors, and a second maintenance stage where the exacerbated pro-oxidant state inside the cell induces the physiological decline characteristic in senescent cells.

Keywords

Senescence P16 P21 P27 Oxidative stress Cell cycle