Biogerontology

, Volume 14, Issue 3, pp 339–352

Expression and modification proteomics during skeletal muscle ageing

Authors

  • Martin A. Baraibar
    • Laboratoire de Biologie Cellulaire du Vieillissement, UR4UPMC Paris 6 University
  • Marine Gueugneau
    • INRA, UMR1019, Centre de Recherche en Nutrition HumaineUniversité d’Auvergne
  • Stephanie Duguez
    • Thérapie des maladies du muscle strié, Institut de Myologie, UMRS 974UPMC Université Paris 6, U974-Inserm, UMR7215-CNRS/AIM
  • Gillian Butler-Browne
    • Thérapie des maladies du muscle strié, Institut de Myologie, UMRS 974UPMC Université Paris 6, U974-Inserm, UMR7215-CNRS/AIM
  • Daniel Bechet
    • INRA, UMR1019, Centre de Recherche en Nutrition HumaineUniversité d’Auvergne
    • Laboratoire de Biologie Cellulaire du Vieillissement, UR4UPMC Paris 6 University
Article

DOI: 10.1007/s10522-013-9426-7

Cite this article as:
Baraibar, M.A., Gueugneau, M., Duguez, S. et al. Biogerontology (2013) 14: 339. doi:10.1007/s10522-013-9426-7

Abstract

Skeletal muscle ageing is characterized by a progressive and dramatic loss of muscle mass and strength leading to decreased muscular function resulting in muscle weakness which is often referred to as sarcopenia. Following the standardisation of “omics” approaches to study the genome (genomics) and the transcriptome (transcriptomics), the study of the proteins encoded by the genome, referred to as proteomics, is a tremendous challenge. Unlike the genome, the proteome varies in response to many physiological or pathological factors. In addition, the proteome is orders of magnitude more complex than the transcriptome due to post-translational modifications, protein oxidation and limited protein degradation. Proteomic studies, including the analysis of protein abundance as well as post-translational modified proteins have been shown to provide valuable information to unravel the key molecular pathways implicated in complex biological processes, such as tissue and organ ageing. In this article, we will describe proteomic approaches for the analysis of protein abundance as well as the specific protein targets for oxidative damage upon oxidative stress and/or during skeletal muscle ageing.

Keywords

2D-gel based expression proteomicsSkeletal muscleAgeingOxidative stressProtein oxidationOxidative proteome modifications

Copyright information

© Springer Science+Business Media Dordrecht 2013