Biogerontology

, Volume 11, Issue 2, pp 183–195

Caenorhabditiselegans lifespan extension caused by treatment with an orally active ROS-generator is dependent on DAF-16 and SIR-2.1

Authors

  • Tanja Heidler
    • Department of Food and Nutrition, Molecular Nutrition UnitTechnical University of Munich
  • Kai Hartwig
    • Department of Food and Nutrition, Molecular Nutrition UnitTechnical University of Munich
  • Hannelore Daniel
    • Department of Food and Nutrition, Molecular Nutrition UnitTechnical University of Munich
    • Molecular Nutrition Research, Interdisciplinary Research CenterJustus-Liebig-University of Giessen
Research Article

DOI: 10.1007/s10522-009-9239-x

Cite this article as:
Heidler, T., Hartwig, K., Daniel, H. et al. Biogerontology (2010) 11: 183. doi:10.1007/s10522-009-9239-x

Abstract

In Caenorhabditiselegans pretreatment with juglone, a generator of reactive oxygen species (ROS) provides a subsequently increased ROS-resistance. We investigated whether juglone at low or high concentrations when provided via the oral route in a liquid axenic medium affects normal lifespan of C.elegans. High juglone concentrations led to premature death, low concentrations were tolerated well and caused a prolongation of lifespan. Lifespan extension under moderate oxidative stress was associated with increased expression of small heat-shock protein HSP-16.2, enhanced glutathione levels, and nuclear translocation of DAF-16. Silencing or deletion of DAF-16 prevented the juglone-induced adaptations. RNA-interference for SIR-2.1 had the same effects as the deletion of DAF-16 but did not affect nuclear accumulation of DAF-16. Our studies demonstrate that DAF-16- and SIR-2.1-dependent alterations in gene expression after a ROS challenge lead to a lifespan extension in C.elegans as long as the stressor concentration does not exceed the saturable protective capacity.

Keywords

CaenorhabditiselegansHormesisReactive oxygen speciesStress responseSmall heat-shock proteinsGlutathione

Copyright information

© Springer Science+Business Media B.V. 2009