Biogerontology

, 10:711

Glyoxalase I activity and immunoreactivity in the aging human lens

Authors

  • Maneesh Mailankot
    • Department of Ophthalmology and Visual SciencesCase Western Reserve University
  • Smitha Padmanabha
    • Department of Ophthalmology and Visual SciencesCase Western Reserve University
  • NagaRekha Pasupuleti
    • Department of Ophthalmology and Visual SciencesCase Western Reserve University
  • Denice Major
    • Visual Sciences Research CenterCase Western Reserve University
  • Scott Howell
    • Visual Sciences Research CenterCase Western Reserve University
    • Department of Ophthalmology and Visual SciencesCase Western Reserve University
Research Article

DOI: 10.1007/s10522-009-9218-2

Cite this article as:
Mailankot, M., Padmanabha, S., Pasupuleti, N. et al. Biogerontology (2009) 10: 711. doi:10.1007/s10522-009-9218-2

Abstract

Glyoxalase I (GLOI) is the first enzyme of the glyoxalase system that catalyzes the metabolism of reactive dicarbonyls, such as methylglyoxal (MGO). During aging and cataract development, human lens proteins are chemically modified by MGO, which is likely due to inadequate metabolism of MGO by the glyoxalase system. In this study, we have determined the effect of aging on GLOI activity and the immunoreactivity and morphological distribution of GLOI in the human lens. A monoclonal antibody was developed against human GLOI. GLOI immunoreactivity was strongest in the anterior epithelial cells and weaker in rest of the lens. Cultured human lens epithelial cells showed immunostaining throughout the cytoplasm. In the human lens, GLOI activity and immunoreactivity both decreased with age. We believe that this would lead to promotion of MGO-modification in aging lens proteins.

Keywords

Glyoxalase IMonoclonal antibodyHuman lensAgingEnzyme activityEnzyme immunoreactivity

Copyright information

© Springer Science+Business Media B.V. 2009