, Volume 10, Issue 5, pp 605–611

Hsp70 prevents disuse muscle atrophy in senescent rats

Research Article

DOI: 10.1007/s10522-008-9203-1

Cite this article as:
Dodd, S., Hain, B. & Judge, A. Biogerontology (2009) 10: 605. doi:10.1007/s10522-008-9203-1


This study determined the effects of heat shock protein 70 (Hsp70) overexpression on disuse muscle atrophy in senescent rats. Solei of young and senescent rats were co-injected with Hsp70 plus a nuclear factor kappa B (NF-κΒ) reporter plasmid. After 4 days, the hind limbs of half the young and senescent rats were immobilized for 6 days with the remainder serving as weight bearing controls. Hsp70 protein levels and cross-sectional area decreased in both groups (~20%) after immobilization. Atrophy was prevented in those fibers overexpressing Hsp70. NF-κΒ activity increased in the soleus of both young (three-fold) and senescent (five-fold) animals after immobilization and was prevented by Hsp70 overexpression. Inhibitor of κΒ decreased in young (~30%) and senescent (~10%) animals with immobilization and returned to normal with Hsp70. Heat shock protein 70 overexpression prevents disuse atrophy in senescent rats, possibly through suppression of the NF-κB pathway.


Heat shock proteinsAgingMuscle wastingNF-κBIκB

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Department of Applied Physiology and KinesiologyUniversity of FloridaGainesvilleUSA