Biogerontology

, Volume 9, Issue 3, pp 183–196

Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver

  • Pilar Caro
  • José Gómez
  • Mónica López-Torres
  • Inés Sánchez
  • Alba Naudí
  • Mariona Jove
  • Reinald Pamplona
  • Gustavo Barja
Research Article

DOI: 10.1007/s10522-008-9130-1

Cite this article as:
Caro, P., Gómez, J., López-Torres, M. et al. Biogerontology (2008) 9: 183. doi:10.1007/s10522-008-9130-1

Abstract

Dietary restriction (DR) lowers mitochondrial reactive oxygen species (ROS) generation and oxidative damage and increases maximum longevity in rodents. Protein restriction (PR) or methionine restriction (MetR), but not lipid or carbohydrate restriction, also cause those kinds of changes. However, previous experiments of MetR were performed only at 80% MetR, and substituting dietary methionine with glutamate in the diet. In order to clarify if MetR can be responsible for the lowered ROS production and oxidative stress induced by standard (40%) DR, Wistar rats were subjected to 40% or 80% MetR without changing other dietary components. It was found that both 40% and 80% MetR decrease mitochondrial ROS generation and percent free radical leak in rat liver mitochondria, similarly to what has been previously observed in 40% PR and 40% DR. The concentration of complexes I and III, apoptosis inducing factor, oxidative damage to mitochondrial DNA, five different markers of protein oxidation, glycoxidation or lipoxidation and fatty acid unsaturation were also lowered. The results show that 40% isocaloric MetR is enough to decrease ROS production and oxidative stress in rat liver. This suggests that the lowered intake of methionine is responsible for the decrease in oxidative stress observed in DR.

Keywords

MitochondriaCaloric restrictionAgingOxygen radicalsLongevityProtein damageFatty acidsRespiratory complexes

Abbreviations

AIF

Apoptosis inducing factor

AASA

Aminoadipic semialdehyde in proteins

CEL

Carboxyethyl-lysine in proteins

CML

Carboxymethyl-lysine in proteins

DBI

Double bond index

DR

Dietary restriction

PI

Peroxidizability index

PR

Protein restriction

GSA

Glutamic semialdehyde in proteins

MDAL

Malondialdehyde-lysine in proteins

MetR

Methionine restriction

ROS

Reactive oxygen species

8-oxodG

8-Oxo-7,8-dihydro-2′deoxyguanosine

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Pilar Caro
    • 1
  • José Gómez
    • 1
  • Mónica López-Torres
    • 1
  • Inés Sánchez
    • 1
  • Alba Naudí
    • 2
  • Mariona Jove
    • 2
  • Reinald Pamplona
    • 2
  • Gustavo Barja
    • 1
  1. 1.Departamento de Fisiología Animal-II, Facultad de Ciencias BiológicasComplutense UniversityMadridSpain
  2. 2.Department of Experimental MedicineUniversity of Lleida-IRBLLEIDALleidaSpain