Research Article

Biogerontology

, Volume 9, Issue 3, pp 183-196

Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver

  • Pilar CaroAffiliated withDepartamento de Fisiología Animal-II, Facultad de Ciencias Biológicas, Complutense University
  • , José GómezAffiliated withDepartamento de Fisiología Animal-II, Facultad de Ciencias Biológicas, Complutense University
  • , Mónica López-TorresAffiliated withDepartamento de Fisiología Animal-II, Facultad de Ciencias Biológicas, Complutense University
  • , Inés SánchezAffiliated withDepartamento de Fisiología Animal-II, Facultad de Ciencias Biológicas, Complutense University
  • , Alba NaudíAffiliated withDepartment of Experimental Medicine, University of Lleida-IRBLLEIDA
  • , Mariona JoveAffiliated withDepartment of Experimental Medicine, University of Lleida-IRBLLEIDA
  • , Reinald PamplonaAffiliated withDepartment of Experimental Medicine, University of Lleida-IRBLLEIDA
  • , Gustavo BarjaAffiliated withDepartamento de Fisiología Animal-II, Facultad de Ciencias Biológicas, Complutense University Email author 

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Abstract

Dietary restriction (DR) lowers mitochondrial reactive oxygen species (ROS) generation and oxidative damage and increases maximum longevity in rodents. Protein restriction (PR) or methionine restriction (MetR), but not lipid or carbohydrate restriction, also cause those kinds of changes. However, previous experiments of MetR were performed only at 80% MetR, and substituting dietary methionine with glutamate in the diet. In order to clarify if MetR can be responsible for the lowered ROS production and oxidative stress induced by standard (40%) DR, Wistar rats were subjected to 40% or 80% MetR without changing other dietary components. It was found that both 40% and 80% MetR decrease mitochondrial ROS generation and percent free radical leak in rat liver mitochondria, similarly to what has been previously observed in 40% PR and 40% DR. The concentration of complexes I and III, apoptosis inducing factor, oxidative damage to mitochondrial DNA, five different markers of protein oxidation, glycoxidation or lipoxidation and fatty acid unsaturation were also lowered. The results show that 40% isocaloric MetR is enough to decrease ROS production and oxidative stress in rat liver. This suggests that the lowered intake of methionine is responsible for the decrease in oxidative stress observed in DR.

Keywords

Mitochondria Caloric restriction Aging Oxygen radicals Longevity Protein damage Fatty acids Respiratory complexes