Biogerontology

, Volume 6, Issue 3, pp 185–192

Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells

Authors

  • Raffaella Moresi
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
  • Silvia Tesei
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
  • Laura Costarelli
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
  • Claudio Viticchi
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
  • Rosalia Stecconi
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
  • Giovanni Bernardini
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
    • Laboratory of Tumor Immunology, INRCA Gerontol. Res. Dept.Immunology Center
Research article

DOI: 10.1007/s10522-005-7954-5

Cite this article as:
Moresi, R., Tesei, S., Costarelli, L. et al. Biogerontology (2005) 6: 185. doi:10.1007/s10522-005-7954-5

Abstract

The aim of this study was to evaluate the peripheral representation and the clonogenic capacity of CD34+ progenitor cells from 130 healthy subjects (80 females and 50 males) ranging in age from 16 to 100 years. We demonstrated that the absolute number of circulating CD34+ cells progressively and significantly decreased with advancing age, with a 2–fold reduction in subjects aged more than 80 years. The number of granulocyte-macrophagic (CFU-GM), erytroid (BFU-E), and mixed (CFU-GEMM) colonies which developed from the number of CD34+ purified cells per ml, progressively and significantly decreased with advancing age. The reduction of both CD34+ cell number and clonogenic capacity during aging was statistically significant in males but not in females. When evaluated on a per cell bases, a significant age-related decrease in the number of CFU-GM colonies was observed in female but not in male subjects. Our study demonstrates the influence of gender on age-related alterations of the number and clonogenic capacity of CD34+ cells in the peripheral blood. This evidence deserves particular consideration for the future planning of stem cell therapy in age-associated debilitating diseases.

Key words

agingcytofluorimetrygenderhumanstem cells

Abbreviations

CFU-GM

colony-forming unit-granulocyte macrophagic

BFU-E

burst-forming unit

CFU-GEMM

colony-forming unit granulocyte-erythroid- macrophagic-megakaryocytic

Copyright information

© Springer 2005