Biogerontology

, Volume 6, Issue 1, pp 15–26

Effect of insulin and growth hormone on rat heart and liver oxidative stress in control and caloric restricted animals

  • A. Sanz
  • R. Gredilla
  • R. Pamplona
  • M. Portero-Otín
  • E. Vara
  • J. A. F. Tresguerres
  • G. Barja
Research article

DOI: 10.1007/s10522-004-7380-0

Cite this article as:
Sanz, A., Gredilla, R., Pamplona, R. et al. Biogerontology (2005) 6: 15. doi:10.1007/s10522-004-7380-0

Abstract

In order to know if insulin-like signalling is involved in the control of oxidative stress in mammalian tissues in relation to aging, ad libitum-fed and caloric restricted Wistar rats were treated during 2 weeks with GH and insulin. The most consistent effect of the hormonal treatments was an increase in plasma IGF-1 levels. Caloric restriction during 6 weeks decreased ROS generation and oxidative DNA damage in heart mitochondria and this was reversed by insulin treatment. The decrease in oxidative damage to liver nuclear DNA induced by caloric restriction was also reversed by GH and insulin. In the liver, however, insulin and GH decreased mitochondrial ROS generation while they increased oxidative damage to mitochondrial DNA. GH and insulin decreased three different markers of oxidative modification of liver proteins, while they increased lipoxidation-dependent markers. This last result is related to the increase in phospholipid unsaturation induced in the liver by both hormones. The results suggest that the idea that insulin-like signalling controls oxidative stress in mammals cannot be generalized since both prooxidant and protective effects of GH and insulin are observed depending on the particular parameter and tissue selected.

Keywords

DNA damage fatty acids free radicals insulin mitochondria protein carbonyls 

Abbreviations

AA

antimycin A

ASA

aminoadipic semialdehyde

CML

Nɛ-(carboxymethyl)lysine

CEL

Nɛ-(carboxyethyl)lysine

DBI

double bond index

dG

deoxyguanosine

FA

fatty acids

GSA

glutamic semialdehyde

MDAL

Nɛ-(malondialdehyde)lysine

mtDNA

mitochondrial DNA

nDNA

nuclear DNA

ROS

reactive oxygen species

ROT

rotenone

8-oxodG

8-oxo,7,8-dihydro-2’-deoxyguanosine

Copyright information

© Springer 2005

Authors and Affiliations

  • A. Sanz
    • 1
  • R. Gredilla
    • 1
  • R. Pamplona
    • 2
  • M. Portero-Otín
    • 2
  • E. Vara
    • 3
  • J. A. F. Tresguerres
    • 4
  • G. Barja
    • 1
  1. 1.Department of Animal Physiology-II, Faculty of BiologyComplutense UniversityMadridSpain
  2. 2.Department of Basic Medical Sciences, Faculty of MedicineUniversity of LleidaLleidaSpain
  3. 3.Department of Biochemistry, Faculty of MedicineComplutense UniversityMadridSpain
  4. 4.Department of Physiology, Faculty of MedicineComplutense UniversityMadridSpain