Biogerontology

, Volume 5, Issue 6, pp 421–429

Vitamin D treatment of senescence accelerated mice (SAM-P/6) induces several regulators of stromal cell plasticity

Research article

DOI: 10.1007/s10522-004-3192-5

Cite this article as:
Duque, G., Macoritto, M. & Kremer, R. Biogerontology (2004) 5: 421. doi:10.1007/s10522-004-3192-5

Abstract

In an attempt to understand the regulation of bone marrow multipotential cells plasticity in vivo, we treated 4-month-old SAM-P/6 mice with a constant infusion of either 18 pmol/24 h of 1,25(OH)2D3 or vehicle alone for 6 weeks. In vehicle treated animals 78% ± 4 adipose volume vs. total volume was stained positive with oil red O as compared to only 32 ± 3% in 1,25(OH)2D3treated animals (P < 0.001). Furthermore, we aimed to identify the changes in gene expression induced by 1,25(OH)2D3in bone marrow cells by analyzing a set of 5440 genes in the NIA 15K Mouse cDNA microarray. Overall, a coordinated regulation of genes which both stimulate osteoblastogenesis and inhibit adipogenesis was observed in 1,25(OH)2D3-treated mice when compared to vehicle treated mice. In summary, this study illustrates the anti-adipogenic effect of 1,25(OH)2D3in bone cells and identifies some of the possible key signals involved in bone cell plasticity.

Keywords

adipogenesismicroarraysosteoblastogenesisSAM-P/6senile osteoporosisvitamin D

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Gustavo Duque
    • 1
    • 2
    • 3
  • Michael Macoritto
    • 3
  • Richard Kremer
    • 3
  1. 1.Division of Geriatric MedicineMcGill UniversityMontrealCanada
  2. 2.Bloomfield Centre for Studies in AgingJewish General HospitalMontrealCanada
  3. 3.Centre for Bone and Periodontal ResearchMcGill UniversityMontrealCanada