Bulletin of Experimental Biology and Medicine

, Volume 157, Issue 2, pp 249–252

Biological Effects of Individually Synthesized TNF-Binding Domain of Variola Virus CrmB Protein

Authors

    • Research Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical Sciences
    • Novosibirsk State Medical UniversityMinistry of Health and Social Development of the Russian Federation
  • I. A. Orlovskaya
    • Research Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical Sciences
  • S. V. Sennikov
    • Research Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical Sciences
  • T. V. Tregubchak
    • Vector State Research CenterKol’tsovo, Novosibirsk Region
  • I. P. Gileva
    • Vector State Research CenterKol’tsovo, Novosibirsk Region
  • M. D. Tsyrendorzhieva
    • Research Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical Sciences
    • Novosibirsk State Medical UniversityMinistry of Health and Social Development of the Russian Federation
  • S. N. Shchelkunov
    • Vector State Research CenterKol’tsovo, Novosibirsk Region
    • Institute of Cytology and GeneticsSiberian Division of the Russian Academy of Sciences
Virology

DOI: 10.1007/s10517-014-2537-6

Cite this article as:
Tsyrendorzhiev, D.D., Orlovskaya, I.A., Sennikov, S.V. et al. Bull Exp Biol Med (2014) 157: 249. doi:10.1007/s10517-014-2537-6

The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the effi ciency of its inhibition of TNF cytotoxicity towards L929 mouse fi broblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.

Keywords

variola virusleukocytestumor necrosis factor-binding proteincytotoxicityoxidative metabolic function

Copyright information

© Springer Science+Business Media New York 2014