Bulletin of Experimental Biology and Medicine

, Volume 157, Issue 2, pp 249–252

Biological Effects of Individually Synthesized TNF-Binding Domain of Variola Virus CrmB Protein

  • D. D. Tsyrendorzhiev
  • I. A. Orlovskaya
  • S. V. Sennikov
  • T. V. Tregubchak
  • I. P. Gileva
  • M. D. Tsyrendorzhieva
  • S. N. Shchelkunov
Virology

DOI: 10.1007/s10517-014-2537-6

Cite this article as:
Tsyrendorzhiev, D.D., Orlovskaya, I.A., Sennikov, S.V. et al. Bull Exp Biol Med (2014) 157: 249. doi:10.1007/s10517-014-2537-6

The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the effi ciency of its inhibition of TNF cytotoxicity towards L929 mouse fi broblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.

Keywords

variola virusleukocytestumor necrosis factor-binding proteincytotoxicityoxidative metabolic function

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • D. D. Tsyrendorzhiev
    • 1
    • 2
  • I. A. Orlovskaya
    • 1
  • S. V. Sennikov
    • 1
  • T. V. Tregubchak
    • 3
  • I. P. Gileva
    • 3
  • M. D. Tsyrendorzhieva
    • 1
    • 2
  • S. N. Shchelkunov
    • 3
    • 4
  1. 1.Research Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical SciencesNovosibirskRussia
  2. 2.Novosibirsk State Medical UniversityMinistry of Health and Social Development of the Russian FederationNovosibirskRussia
  3. 3.Vector State Research CenterKol’tsovo, Novosibirsk RegionNovosibirskRussia
  4. 4.Institute of Cytology and GeneticsSiberian Division of the Russian Academy of SciencesNovosibirskRussia