Bulletin of Experimental Biology and Medicine

, Volume 156, Issue 3, pp 393–398

Morphological and Phenotypical Characteristics of Human Osteoblasts after Short-Term Space Mission

Authors

  • M. Yu. Kapitonova
    • Faculty of MedicineUniversiti Teknologi MARA
    • Institute of Medical Molecular Biotechnology
  • S. L. Kuznetsov
    • I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
  • N. Salim
    • Institute of Medical Molecular Biotechnology
  • S. Othman
    • Institute of Medical Molecular Biotechnology
  • T. M. H. T. M. Kamauzaman
    • Institute of Medical Molecular Biotechnology
  • A. M. Ali
    • Faculty of Agriculture and BiotechnologyUniversiti Sultan Zainal Abidin
  • H. M. Nawawi
    • Faculty of MedicineUniversiti Teknologi MARA
  • M. N. K. Nor-Ashikin
    • Faculty of MedicineUniversiti Teknologi MARA
    • Institute of Medical Molecular Biotechnology
MORPHOLOGY AND PATHOMORPHOLOGY

DOI: 10.1007/s10517-014-2357-8

Cite this article as:
Kapitonova, M.Y., Kuznetsov, S.L., Salim, N. et al. Bull Exp Biol Med (2014) 156: 393. doi:10.1007/s10517-014-2357-8

Morphological and phenotypical signs of cultured readaptation osteoblasts were studied after a short-term space mission. The ultrastructure and phenotype of human osteoblasts after Soyuz TMA-11 space flight (2007) were evaluated by scanning electron microscopy, laser confocal microscopy, and ELISA. The morphofunctional changes in cell cultures persisted after 12 passages. Osteoblasts retained the drastic changes in their shape and size, contour deformation, disorganization of the microtubular network, redistribution of organelles and specialized structures of the plasmalemma in comparison with the ground control cells. On the other hand, the expression of osteoprotegerin and osteocalcin (bone metabolism markers) increased; the expression of bone resorption markers ICAM-1 and IL-6 also increased, while the expression of VCAM-1 decreased. Hence, space flight led to the development of persistent shifts in cultured osteoblasts indicating injuries to the cytoskeleton and the phenotype changes, indicating modulation of bone metabolism biomarkers.

Key Words

osteoblastcell culturemicrogravitationfluorescent microscopyscanning electron microscopy

Copyright information

© Springer Science+Business Media New York 2014