Bulletin of Experimental Biology and Medicine

, Volume 150, Issue 1, pp 157–159

Resazurin Dye as a Reliable Tool for Determination of Cell Number and Viability in Mesenchymal Stem Cell Culture

  • T. Dienstknecht
  • K. Ehehalt
  • Zsuzsa Jenei-Lanzl
  • J. Zellner
  • Michael Müller
  • A. Berner
  • M. Nerlich
  • P. Angele
Article

DOI: 10.1007/s10517-010-1092-z

Cite this article as:
Dienstknecht, T., Ehehalt, K., Jenei-Lanzl, Z. et al. Bull Exp Biol Med (2010) 150: 157. doi:10.1007/s10517-010-1092-z

Human mesenchymal stem cells are a valuable cell source for tissue engineering. Determination of cell number and viability is crucial. However, this can be tested only at the end of cell culture. This study shows that Resazurin dye staining is a reliable tool for evaluation of cell number and viability in culture without cell perturbation.

Key Words

Resazurin dyemesenchymal stem cellscell count

Copyright information

© Springer Science+Business Media, Inc. 2010

Authors and Affiliations

  • T. Dienstknecht
    • 1
  • K. Ehehalt
    • 1
  • Zsuzsa Jenei-Lanzl
    • 1
  • J. Zellner
    • 1
  • Michael Müller
    • 1
  • A. Berner
    • 1
  • M. Nerlich
    • 1
  • P. Angele
    • 1
  1. 1.Department for TraumatologyUniversity Hospital RegensburgRegensburgGermany