Antiaggregant Effect of Taurine Chloramines in the Presence of Serum Albumin

  • M. A. Murina
  • D. I. Roshchupkin
  • N. A. Chudina
  • A. O. Petrova
  • V. I. Sergienko
Article

DOI: 10.1007/s10517-009-0601-4

Cite this article as:
Murina, M.A., Roshchupkin, D.I., Chudina, N.A. et al. Bull Exp Biol Med (2009) 147: 704. doi:10.1007/s10517-009-0601-4

The effects of taurine chloramine derivatives on initial aggregation of isolated platelets suspended in buffered saline were studied. Inhibition of ADP-induced aggregation in pure cell suspension depended on the structure of chloramine antiaggregants. The most effective of them was N,N-dichlorotaurine; its concentration needed for 50% inhibition of aggregation was about 0.1 mM. Weaker antiaggregants N-chloro-N-methyltaurine and N-chlorotaurine in a final concentration of 0.5 mM reduced platelet aggregation by only 10%. The studied chloramines considerably differed by their characteristics (velocity of the reaction with sulfur-containing groups of atoms). N,N-dichlorotaurine exhibited the weakest reactivity with methionine thioester group. In turn, the velocity constant with reduced glutathione was by 2-3 orders of magnitude higher than that of other chloramines. Antiaggregant effect of taurine chloramine derivatives was 2-fold higher in the presence of serum albumin, presumably due to special interactions of taurine chloramines in complex with albumin with platelets.

Key Words

chloramineN,N-dichlorotaurineserum albuminplateletsaggregation

Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • M. A. Murina
    • 1
  • D. I. Roshchupkin
    • 2
  • N. A. Chudina
    • 1
  • A. O. Petrova
    • 1
  • V. I. Sergienko
    • 1
  1. 1.Federal Agency for Health Care and Social DevelopmentInstitute of Physicochemical MedicineMoscowRussia
  2. 2.Federal Agency for Health Care and Social DevelopmentRussian State Medical UniversityMoscowRussia