Bulletin of Experimental Biology and Medicine

, Volume 147, Issue 2, pp 220–225

Hepatitis B Virus Genetic Typing Using Mass-Spectrometry

  • M. V. Malakhova
  • E. N. Ilina
  • V. M. Govorun
  • S. A. Shutko
  • K. R. Dudina
  • O. O. Znoyko
  • E. A. Klimova
  • N. D. Iushchuk
Virology

DOI: 10.1007/s10517-009-0479-1

Cite this article as:
Malakhova, M.V., Ilina, E.N., Govorun, V.M. et al. Bull Exp Biol Med (2009) 147: 220. doi:10.1007/s10517-009-0479-1

Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing.

Key Words

viral hepatitis Bgenetic typingmini-sequencingmass-spectrometry

Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • M. V. Malakhova
    • 1
  • E. N. Ilina
    • 1
  • V. M. Govorun
    • 1
  • S. A. Shutko
    • 2
  • K. R. Dudina
    • 2
  • O. O. Znoyko
    • 2
  • E. A. Klimova
    • 2
  • N. D. Iushchuk
    • 2
  1. 1.Scientific Research Institute of Physical-Chemical MedicineMoscowRussia
  2. 2.Moscow State Medical-Stomatological UniversityMoscowRussia