, Volume 146, Issue 4, pp 466-469
Date: 11 Feb 2009

Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise

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We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 10, pp. 446–450, October, 2008